Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS04085

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS04075 and ECOLIN_RS04080 are separated by 0 nucleotidesECOLIN_RS04080 and ECOLIN_RS04085 are separated by 154 nucleotidesECOLIN_RS04085 and ECOLIN_RS04090 are separated by 40 nucleotides ECOLIN_RS04075: ECOLIN_RS04075 - molybdenum ABC transporter ATP-binding protein ModC, at 823,241 to 824,299 _RS04075 ECOLIN_RS04080: ECOLIN_RS04080 - bifunctional pyridoxal phosphate/fructose-1,6-bisphosphate phosphatase, at 824,300 to 825,118 _RS04080 ECOLIN_RS04085: ECOLIN_RS04085 - 6-phosphogluconolactonase, at 825,273 to 826,268 _RS04085 ECOLIN_RS04090: ECOLIN_RS04090 - LysR family transcriptional regulator, at 826,309 to 827,262 _RS04090 Position (kb) 825 826 827Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 824.602 kb on + strand, within ECOLIN_RS04080at 824.663 kb on - strand, within ECOLIN_RS04080at 824.778 kb on + strand, within ECOLIN_RS04080at 824.800 kb on - strand, within ECOLIN_RS04080at 825.530 kb on + strand, within ECOLIN_RS04085at 825.573 kb on + strand, within ECOLIN_RS04085at 825.573 kb on + strand, within ECOLIN_RS04085at 825.689 kb on + strand, within ECOLIN_RS04085at 825.971 kb on + strand, within ECOLIN_RS04085at 826.201 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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824,602 + ECOLIN_RS04080 0.37 +0.0
824,663 - ECOLIN_RS04080 0.44 -0.3
824,778 + ECOLIN_RS04080 0.58 -0.4
824,800 - ECOLIN_RS04080 0.61 -0.2
825,530 + ECOLIN_RS04085 0.26 -4.5
825,573 + ECOLIN_RS04085 0.30 -3.3
825,573 + ECOLIN_RS04085 0.30 -4.8
825,689 + ECOLIN_RS04085 0.42 -2.4
825,971 + ECOLIN_RS04085 0.70 -3.6
826,201 + -1.3

Or see this region's nucleotide sequence