Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS03870

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS03860 and ECOLIN_RS03870 are separated by 550 nucleotidesECOLIN_RS03870 and ECOLIN_RS03875 are separated by 14 nucleotides ECOLIN_RS03860: ECOLIN_RS03860 - succinate dehydrogenase iron-sulfur subunit SdhB, at 789,122 to 789,838 _RS03860 ECOLIN_RS03870: ECOLIN_RS03870 - 2-oxoglutarate dehydrogenase E1 component, at 790,389 to 793,190 _RS03870 ECOLIN_RS03875: ECOLIN_RS03875 - 2-oxoglutarate dehydrogenase complex dihydrolipoyllysine-residue succinyltransferase, at 793,205 to 794,422 _RS03875 Position (kb) 790 791 792 793 794Strain fitness (log2 ratio) -1 0 1 2 3at 789.435 kb on + strand, within ECOLIN_RS03860at 789.522 kb on + strand, within ECOLIN_RS03860at 789.829 kb on + strandat 789.829 kb on + strandat 789.829 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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789,435 + ECOLIN_RS03860 0.44 +0.5
789,522 + ECOLIN_RS03860 0.56 +3.7
789,829 + +1.0
789,829 + -1.2
789,829 + +0.0

Or see this region's nucleotide sequence