Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS03620

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS03615 and ECOLIN_RS03620 are separated by 59 nucleotidesECOLIN_RS03620 and ECOLIN_RS03625 are separated by 332 nucleotides ECOLIN_RS03615: ECOLIN_RS03615 - N-acetylglucosamine-6-phosphate deacetylase, at 739,552 to 740,700 _RS03615 ECOLIN_RS03620: ECOLIN_RS03620 - glucosamine-6-phosphate deaminase, at 740,760 to 741,560 _RS03620 ECOLIN_RS03625: ECOLIN_RS03625 - PTS N-acetyl glucosamine transporter subunit IIABC, at 741,893 to 743,839 _RS03625 Position (kb) 740 741 742Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 739.966 kb on - strand, within ECOLIN_RS03615at 740.034 kb on - strand, within ECOLIN_RS03615at 740.422 kb on - strand, within ECOLIN_RS03615at 740.422 kb on - strand, within ECOLIN_RS03615at 740.425 kb on - strand, within ECOLIN_RS03615at 740.697 kb on - strandat 740.697 kb on - strandat 740.697 kb on - strandat 740.697 kb on - strandat 740.697 kb on - strandat 740.697 kb on - strandat 741.132 kb on - strand, within ECOLIN_RS03620at 741.616 kb on + strandat 741.869 kb on + strandat 742.000 kb on + strandat 742.000 kb on + strandat 742.130 kb on + strand, within ECOLIN_RS03625at 742.174 kb on + strand, within ECOLIN_RS03625

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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739,966 - ECOLIN_RS03615 0.36 -0.9
740,034 - ECOLIN_RS03615 0.42 +2.7
740,422 - ECOLIN_RS03615 0.76 -2.3
740,422 - ECOLIN_RS03615 0.76 -1.0
740,425 - ECOLIN_RS03615 0.76 -5.9
740,697 - -2.8
740,697 - -2.7
740,697 - -2.4
740,697 - -0.4
740,697 - -2.6
740,697 - -2.6
741,132 - ECOLIN_RS03620 0.46 -0.5
741,616 + +1.7
741,869 + -0.2
742,000 + -3.3
742,000 + +0.1
742,130 + ECOLIN_RS03625 0.12 -0.4
742,174 + ECOLIN_RS03625 0.14 -5.7

Or see this region's nucleotide sequence