Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS19505

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS19495 and ECOLIN_RS19500 are separated by 58 nucleotidesECOLIN_RS19500 and ECOLIN_RS19505 are separated by 363 nucleotidesECOLIN_RS19505 and ECOLIN_RS19510 are separated by 110 nucleotides ECOLIN_RS19495: ECOLIN_RS19495 - DNA utilization protein GntX, at 3,900,877 to 3,901,560 _RS19495 ECOLIN_RS19500: ECOLIN_RS19500 - Fe-S biogenesis protein NfuA, at 3,901,619 to 3,902,194 _RS19500 ECOLIN_RS19505: ECOLIN_RS19505 - gluconate transporter, at 3,902,558 to 3,903,874 _RS19505 ECOLIN_RS19510: ECOLIN_RS19510 - 4-alpha-glucanotransferase, at 3,903,985 to 3,906,069 _RS19510 Position (kb) 3902 3903 3904Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 3901.618 kb on + strandat 3901.627 kb on + strandat 3901.808 kb on + strand, within ECOLIN_RS19500at 3901.808 kb on + strand, within ECOLIN_RS19500at 3901.808 kb on + strand, within ECOLIN_RS19500at 3901.821 kb on + strand, within ECOLIN_RS19500at 3901.955 kb on + strand, within ECOLIN_RS19500at 3901.955 kb on + strand, within ECOLIN_RS19500at 3901.956 kb on - strand, within ECOLIN_RS19500at 3901.956 kb on - strand, within ECOLIN_RS19500at 3901.956 kb on - strand, within ECOLIN_RS19500at 3901.956 kb on - strand, within ECOLIN_RS19500at 3901.956 kb on - strand, within ECOLIN_RS19500at 3901.956 kb on - strand, within ECOLIN_RS19500at 3902.186 kb on + strandat 3902.187 kb on - strandat 3902.187 kb on - strandat 3902.187 kb on - strandat 3902.189 kb on + strandat 3902.232 kb on - strandat 3902.252 kb on + strandat 3902.253 kb on - strandat 3902.286 kb on + strandat 3902.287 kb on - strandat 3902.326 kb on - strandat 3902.473 kb on - strandat 3902.595 kb on + strandat 3902.595 kb on + strandat 3902.696 kb on - strand, within ECOLIN_RS19505at 3902.863 kb on - strand, within ECOLIN_RS19505at 3902.970 kb on - strand, within ECOLIN_RS19505at 3902.970 kb on - strand, within ECOLIN_RS19505at 3903.087 kb on - strand, within ECOLIN_RS19505at 3903.094 kb on + strand, within ECOLIN_RS19505at 3903.095 kb on - strand, within ECOLIN_RS19505at 3903.527 kb on + strand, within ECOLIN_RS19505at 3903.527 kb on + strand, within ECOLIN_RS19505at 3903.527 kb on + strand, within ECOLIN_RS19505at 3903.527 kb on + strand, within ECOLIN_RS19505at 3903.768 kb on - strandat 3903.878 kb on + strandat 3903.935 kb on - strandat 3904.126 kb on - strandat 3904.284 kb on + strand, within ECOLIN_RS19510at 3904.662 kb on - strand, within ECOLIN_RS19510at 3904.662 kb on - strand, within ECOLIN_RS19510at 3904.662 kb on - strand, within ECOLIN_RS19510at 3904.788 kb on - strand, within ECOLIN_RS19510at 3904.788 kb on - strand, within ECOLIN_RS19510

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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3,901,618 + -0.7
3,901,627 + -3.9
3,901,808 + ECOLIN_RS19500 0.33 -3.7
3,901,808 + ECOLIN_RS19500 0.33 -2.7
3,901,808 + ECOLIN_RS19500 0.33 -6.0
3,901,821 + ECOLIN_RS19500 0.35 -4.4
3,901,955 + ECOLIN_RS19500 0.58 -3.6
3,901,955 + ECOLIN_RS19500 0.58 -3.2
3,901,956 - ECOLIN_RS19500 0.59 -4.0
3,901,956 - ECOLIN_RS19500 0.59 -3.9
3,901,956 - ECOLIN_RS19500 0.59 -2.3
3,901,956 - ECOLIN_RS19500 0.59 -3.0
3,901,956 - ECOLIN_RS19500 0.59 -3.7
3,901,956 - ECOLIN_RS19500 0.59 -3.2
3,902,186 + -0.9
3,902,187 - -1.0
3,902,187 - -1.0
3,902,187 - -0.9
3,902,189 + -1.1
3,902,232 - -0.1
3,902,252 + +0.5
3,902,253 - +0.5
3,902,286 + +0.9
3,902,287 - +0.2
3,902,326 - +0.2
3,902,473 - +0.4
3,902,595 + +0.1
3,902,595 + -0.4
3,902,696 - ECOLIN_RS19505 0.10 -1.0
3,902,863 - ECOLIN_RS19505 0.23 +0.2
3,902,970 - ECOLIN_RS19505 0.31 +0.2
3,902,970 - ECOLIN_RS19505 0.31 +0.0
3,903,087 - ECOLIN_RS19505 0.40 +0.8
3,903,094 + ECOLIN_RS19505 0.41 +0.2
3,903,095 - ECOLIN_RS19505 0.41 -0.0
3,903,527 + ECOLIN_RS19505 0.74 -0.3
3,903,527 + ECOLIN_RS19505 0.74 +0.1
3,903,527 + ECOLIN_RS19505 0.74 +0.0
3,903,527 + ECOLIN_RS19505 0.74 -0.0
3,903,768 - -0.2
3,903,878 + +0.1
3,903,935 - -0.9
3,904,126 - -3.8
3,904,284 + ECOLIN_RS19510 0.14 -6.6
3,904,662 - ECOLIN_RS19510 0.32 -4.7
3,904,662 - ECOLIN_RS19510 0.32 -4.4
3,904,662 - ECOLIN_RS19510 0.32 -4.0
3,904,788 - ECOLIN_RS19510 0.39 -3.3
3,904,788 - ECOLIN_RS19510 0.39 -4.9

Or see this region's nucleotide sequence