Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS18040

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS18030 and ECOLIN_RS18035 are separated by 10 nucleotidesECOLIN_RS18035 and ECOLIN_RS18040 overlap by 11 nucleotidesECOLIN_RS18040 and ECOLIN_RS18045 are separated by 17 nucleotidesECOLIN_RS18045 and ECOLIN_RS18050 overlap by 4 nucleotides ECOLIN_RS18030: ECOLIN_RS18030 - PTS N-acetylgalactosamine transporter subunit IIB, at 3,636,576 to 3,637,049 _RS18030 ECOLIN_RS18035: ECOLIN_RS18035 - PTS N-acetylgalactosamine transporter subunit IIC, at 3,637,060 to 3,637,839 _RS18035 ECOLIN_RS18040: ECOLIN_RS18040 - PTS N-acetylgalactosamine transporter subunit IID, at 3,637,829 to 3,638,707 _RS18040 ECOLIN_RS18045: ECOLIN_RS18045 - PTS galactosamine/N-acetylgalactosamine transporter subunit IIA, at 3,638,725 to 3,639,159 _RS18045 ECOLIN_RS18050: ECOLIN_RS18050 - N-acetylglucosamine-6-phosphate deacetylase, at 3,639,156 to 3,640,289 _RS18050 Position (kb) 3637 3638 3639Strain fitness (log2 ratio) -1 0 1 2at 3637.207 kb on - strand, within ECOLIN_RS18035at 3637.318 kb on - strand, within ECOLIN_RS18035at 3637.389 kb on + strand, within ECOLIN_RS18035at 3637.389 kb on + strand, within ECOLIN_RS18035at 3637.389 kb on + strand, within ECOLIN_RS18035at 3637.510 kb on + strand, within ECOLIN_RS18035at 3637.533 kb on + strand, within ECOLIN_RS18035at 3637.672 kb on + strand, within ECOLIN_RS18035at 3637.907 kb on + strandat 3637.908 kb on - strandat 3637.908 kb on - strandat 3637.948 kb on + strand, within ECOLIN_RS18040at 3638.005 kb on + strand, within ECOLIN_RS18040at 3638.065 kb on + strand, within ECOLIN_RS18040at 3638.149 kb on + strand, within ECOLIN_RS18040at 3638.171 kb on - strand, within ECOLIN_RS18040at 3638.259 kb on + strand, within ECOLIN_RS18040at 3638.298 kb on + strand, within ECOLIN_RS18040at 3638.455 kb on + strand, within ECOLIN_RS18040at 3638.455 kb on + strand, within ECOLIN_RS18040at 3638.455 kb on + strand, within ECOLIN_RS18040at 3638.455 kb on + strand, within ECOLIN_RS18040at 3638.456 kb on - strand, within ECOLIN_RS18040at 3638.495 kb on + strand, within ECOLIN_RS18040at 3638.495 kb on + strand, within ECOLIN_RS18040at 3638.495 kb on + strand, within ECOLIN_RS18040at 3638.550 kb on + strand, within ECOLIN_RS18040at 3638.601 kb on - strand, within ECOLIN_RS18040at 3638.720 kb on - strandat 3638.722 kb on + strandat 3638.856 kb on + strand, within ECOLIN_RS18045at 3638.856 kb on + strand, within ECOLIN_RS18045at 3638.856 kb on + strand, within ECOLIN_RS18045at 3638.857 kb on - strand, within ECOLIN_RS18045at 3638.857 kb on - strand, within ECOLIN_RS18045at 3639.238 kb on + strandat 3639.322 kb on + strand, within ECOLIN_RS18050at 3639.323 kb on - strand, within ECOLIN_RS18050at 3639.347 kb on - strand, within ECOLIN_RS18050at 3639.470 kb on + strand, within ECOLIN_RS18050at 3639.537 kb on + strand, within ECOLIN_RS18050at 3639.696 kb on - strand, within ECOLIN_RS18050

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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3,637,207 - ECOLIN_RS18035 0.19 -0.5
3,637,318 - ECOLIN_RS18035 0.33 +0.2
3,637,389 + ECOLIN_RS18035 0.42 +1.9
3,637,389 + ECOLIN_RS18035 0.42 +0.8
3,637,389 + ECOLIN_RS18035 0.42 -0.5
3,637,510 + ECOLIN_RS18035 0.58 +0.2
3,637,533 + ECOLIN_RS18035 0.61 +0.1
3,637,672 + ECOLIN_RS18035 0.78 +0.5
3,637,907 + +0.7
3,637,908 - +0.1
3,637,908 - -0.3
3,637,948 + ECOLIN_RS18040 0.14 +0.3
3,638,005 + ECOLIN_RS18040 0.20 +0.0
3,638,065 + ECOLIN_RS18040 0.27 +0.4
3,638,149 + ECOLIN_RS18040 0.36 -0.9
3,638,171 - ECOLIN_RS18040 0.39 -0.4
3,638,259 + ECOLIN_RS18040 0.49 -0.3
3,638,298 + ECOLIN_RS18040 0.53 -0.3
3,638,455 + ECOLIN_RS18040 0.71 -0.2
3,638,455 + ECOLIN_RS18040 0.71 +0.3
3,638,455 + ECOLIN_RS18040 0.71 -0.0
3,638,455 + ECOLIN_RS18040 0.71 -0.3
3,638,456 - ECOLIN_RS18040 0.71 +0.5
3,638,495 + ECOLIN_RS18040 0.76 +0.1
3,638,495 + ECOLIN_RS18040 0.76 -1.0
3,638,495 + ECOLIN_RS18040 0.76 +0.1
3,638,550 + ECOLIN_RS18040 0.82 +0.0
3,638,601 - ECOLIN_RS18040 0.88 -0.6
3,638,720 - -0.5
3,638,722 + +0.9
3,638,856 + ECOLIN_RS18045 0.30 -0.0
3,638,856 + ECOLIN_RS18045 0.30 +0.3
3,638,856 + ECOLIN_RS18045 0.30 -0.0
3,638,857 - ECOLIN_RS18045 0.30 -0.3
3,638,857 - ECOLIN_RS18045 0.30 +0.6
3,639,238 + +0.4
3,639,322 + ECOLIN_RS18050 0.15 +0.0
3,639,323 - ECOLIN_RS18050 0.15 +0.2
3,639,347 - ECOLIN_RS18050 0.17 -0.3
3,639,470 + ECOLIN_RS18050 0.28 -0.1
3,639,537 + ECOLIN_RS18050 0.34 -0.1
3,639,696 - ECOLIN_RS18050 0.48 -0.7

Or see this region's nucleotide sequence