Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12725

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12720 and ECOLIN_RS12725 are separated by 180 nucleotidesECOLIN_RS12725 and ECOLIN_RS12730 are separated by 81 nucleotides ECOLIN_RS12720: ECOLIN_RS12720 - bifunctional murein DD-endopeptidase/murein LD-carboxypeptidase, at 2,532,569 to 2,533,135 _RS12720 ECOLIN_RS12725: ECOLIN_RS12725 - cyclic di-GMP phosphodiesterase, at 2,533,316 to 2,534,872 _RS12725 ECOLIN_RS12730: ECOLIN_RS12730 - extracellular solute-binding protein, at 2,534,954 to 2,536,768 _RS12730 Position (kb) 2533 2534 2535Strain fitness (log2 ratio) -2 -1 0 1 2at 2532.436 kb on + strandat 2532.598 kb on + strandat 2532.649 kb on + strand, within ECOLIN_RS12720at 2532.969 kb on + strand, within ECOLIN_RS12720at 2533.013 kb on + strand, within ECOLIN_RS12720at 2533.013 kb on + strand, within ECOLIN_RS12720at 2533.063 kb on + strand, within ECOLIN_RS12720at 2533.533 kb on + strand, within ECOLIN_RS12725at 2533.696 kb on + strand, within ECOLIN_RS12725at 2535.713 kb on + strand, within ECOLIN_RS12730

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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2,532,436 + -1.1
2,532,598 + -1.9
2,532,649 + ECOLIN_RS12720 0.14 -0.3
2,532,969 + ECOLIN_RS12720 0.71 -0.3
2,533,013 + ECOLIN_RS12720 0.78 -1.4
2,533,013 + ECOLIN_RS12720 0.78 -1.0
2,533,063 + ECOLIN_RS12720 0.87 -0.2
2,533,533 + ECOLIN_RS12725 0.14 +0.1
2,533,696 + ECOLIN_RS12725 0.24 +0.8
2,535,713 + ECOLIN_RS12730 0.42 +2.6

Or see this region's nucleotide sequence