Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS03605

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS03600 and ECOLIN_RS03605 are separated by 255 nucleotidesECOLIN_RS03605 and ECOLIN_RS03610 are separated by 47 nucleotides ECOLIN_RS03600: ECOLIN_RS03600 - asparagine synthase B, at 735,603 to 737,267 _RS03600 ECOLIN_RS03605: ECOLIN_RS03605 - ribonucleotide monophosphatase NagD, at 737,523 to 738,275 _RS03605 ECOLIN_RS03610: ECOLIN_RS03610 - DNA-binding transcriptional regulator NagC, at 738,323 to 739,543 _RS03610 Position (kb) 737 738 739Strain fitness (log2 ratio) -2 -1 0 1at 736.798 kb on + strand, within ECOLIN_RS03600at 736.828 kb on + strand, within ECOLIN_RS03600at 736.828 kb on + strand, within ECOLIN_RS03600at 737.267 kb on + strandat 737.267 kb on + strandat 737.579 kb on + strandat 737.580 kb on - strandat 737.759 kb on - strand, within ECOLIN_RS03605at 737.759 kb on - strand, within ECOLIN_RS03605at 737.882 kb on - strand, within ECOLIN_RS03605at 737.978 kb on - strand, within ECOLIN_RS03605at 737.978 kb on - strand, within ECOLIN_RS03605at 738.014 kb on - strand, within ECOLIN_RS03605at 738.301 kb on + strandat 738.431 kb on + strandat 738.437 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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736,798 + ECOLIN_RS03600 0.72 -1.1
736,828 + ECOLIN_RS03600 0.74 -0.4
736,828 + ECOLIN_RS03600 0.74 +0.2
737,267 + +0.7
737,267 + +0.9
737,579 + -0.0
737,580 - -0.1
737,759 - ECOLIN_RS03605 0.31 -0.2
737,759 - ECOLIN_RS03605 0.31 -2.2
737,882 - ECOLIN_RS03605 0.48 +0.2
737,978 - ECOLIN_RS03605 0.60 +0.4
737,978 - ECOLIN_RS03605 0.60 +0.7
738,014 - ECOLIN_RS03605 0.65 +1.1
738,301 + -0.2
738,431 + +0.1
738,437 - -1.3

Or see this region's nucleotide sequence