Experiment: MoYLS4 with Spectinomycin 0.0125mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0599 and ldh are separated by 297 nucleotides ldh and b1396 are separated by 411 nucleotides b1396 and tRNA-Glu are separated by 143 nucleotides
DVU0599: DVU0599 - carbon starvation protein A, putative (TIGR), at 669,038 to 670,459
DVU0599
DVU0600: ldh - L-lactate dehydrogenase (TIGR), at 670,757 to 671,686
ldh
DVU0601: b1396 - phenylacetic acid degradation protein PaaI (TIGR), at 672,098 to 672,532
b1396
DVU_tRNA-Glu-1: tRNA-Glu - tRNA with anticodon TTC for Glu (VIMSS-AUTO), at 672,676 to 672,752
tRNA-Glu
Position (kb) 670
671
672 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 670.102 kb on + strand, within DVU0599 at 670.157 kb on - strand, within DVU0599 at 670.454 kb on + strand at 670.462 kb on - strand at 670.468 kb on - strand at 670.527 kb on + strand at 670.542 kb on + strand at 670.547 kb on - strand at 670.552 kb on + strand at 670.560 kb on - strand at 670.560 kb on - strand at 670.622 kb on + strand at 670.640 kb on - strand at 670.706 kb on - strand at 670.712 kb on - strand at 670.742 kb on + strand at 670.759 kb on + strand at 670.839 kb on + strand at 670.855 kb on + strand, within ldh at 670.863 kb on - strand, within ldh at 670.876 kb on + strand, within ldh at 670.902 kb on - strand, within ldh at 670.930 kb on - strand, within ldh at 670.941 kb on + strand, within ldh at 670.951 kb on + strand, within ldh at 670.953 kb on + strand, within ldh at 671.037 kb on - strand, within ldh at 671.074 kb on - strand, within ldh at 671.128 kb on - strand, within ldh at 671.128 kb on - strand, within ldh at 671.186 kb on + strand, within ldh at 671.194 kb on - strand, within ldh at 671.232 kb on + strand, within ldh at 671.256 kb on + strand, within ldh at 671.281 kb on + strand, within ldh at 671.291 kb on + strand, within ldh at 671.384 kb on + strand, within ldh at 671.384 kb on + strand, within ldh at 671.384 kb on + strand, within ldh at 671.384 kb on + strand, within ldh at 671.433 kb on + strand, within ldh at 671.441 kb on + strand, within ldh at 671.462 kb on - strand, within ldh at 671.462 kb on - strand, within ldh at 671.467 kb on - strand, within ldh at 671.478 kb on + strand, within ldh at 671.515 kb on + strand, within ldh at 671.592 kb on + strand, within ldh at 671.680 kb on + strand at 671.688 kb on - strand at 671.704 kb on - strand at 671.735 kb on + strand at 671.738 kb on - strand at 671.870 kb on + strand at 671.994 kb on + strand at 672.072 kb on + strand at 672.072 kb on + strand at 672.082 kb on + strand at 672.149 kb on - strand, within b1396 at 672.185 kb on + strand, within b1396 at 672.193 kb on - strand, within b1396 at 672.230 kb on + strand, within b1396 at 672.231 kb on - strand, within b1396 at 672.231 kb on - strand, within b1396 at 672.231 kb on - strand, within b1396 at 672.272 kb on + strand, within b1396 at 672.336 kb on + strand, within b1396 at 672.416 kb on + strand, within b1396 at 672.422 kb on - strand, within b1396 at 672.435 kb on + strand, within b1396 at 672.522 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.0125mg/mL remove 670,102 + DVU0599 0.75 -1.2 670,157 - DVU0599 0.79 -0.2 670,454 + -0.5 670,462 - +0.1 670,468 - +0.4 670,527 + -0.1 670,542 + -0.6 670,547 - +0.2 670,552 + -0.1 670,560 - -0.7 670,560 - +0.4 670,622 + -0.1 670,640 - +0.3 670,706 - +1.1 670,712 - -0.0 670,742 + -0.4 670,759 + -0.6 670,839 + +0.0 670,855 + ldh DVU0600 0.11 +0.2 670,863 - ldh DVU0600 0.11 -1.0 670,876 + ldh DVU0600 0.13 +0.5 670,902 - ldh DVU0600 0.16 -0.5 670,930 - ldh DVU0600 0.19 -0.6 670,941 + ldh DVU0600 0.20 -0.6 670,951 + ldh DVU0600 0.21 +0.3 670,953 + ldh DVU0600 0.21 -0.9 671,037 - ldh DVU0600 0.30 +0.2 671,074 - ldh DVU0600 0.34 +0.1 671,128 - ldh DVU0600 0.40 -0.1 671,128 - ldh DVU0600 0.40 -0.3 671,186 + ldh DVU0600 0.46 -1.9 671,194 - ldh DVU0600 0.47 +0.2 671,232 + ldh DVU0600 0.51 -1.1 671,256 + ldh DVU0600 0.54 -0.6 671,281 + ldh DVU0600 0.56 -0.7 671,291 + ldh DVU0600 0.57 -0.1 671,384 + ldh DVU0600 0.67 -0.3 671,384 + ldh DVU0600 0.67 -0.4 671,384 + ldh DVU0600 0.67 +2.5 671,384 + ldh DVU0600 0.67 -0.3 671,433 + ldh DVU0600 0.73 -0.5 671,441 + ldh DVU0600 0.74 -0.6 671,462 - ldh DVU0600 0.76 -0.7 671,462 - ldh DVU0600 0.76 +0.1 671,467 - ldh DVU0600 0.76 +0.0 671,478 + ldh DVU0600 0.78 -0.4 671,515 + ldh DVU0600 0.82 +4.1 671,592 + ldh DVU0600 0.90 -0.4 671,680 + +0.2 671,688 - +1.4 671,704 - +0.2 671,735 + -0.7 671,738 - +0.5 671,870 + +0.7 671,994 + -0.3 672,072 + -0.1 672,072 + -0.3 672,082 + -0.2 672,149 - b1396 DVU0601 0.12 -0.6 672,185 + b1396 DVU0601 0.20 +0.0 672,193 - b1396 DVU0601 0.22 +0.4 672,230 + b1396 DVU0601 0.30 -0.8 672,231 - b1396 DVU0601 0.31 -0.3 672,231 - b1396 DVU0601 0.31 +0.1 672,231 - b1396 DVU0601 0.31 -0.9 672,272 + b1396 DVU0601 0.40 +0.5 672,336 + b1396 DVU0601 0.55 +0.1 672,416 + b1396 DVU0601 0.73 -0.2 672,422 - b1396 DVU0601 0.74 +0.1 672,435 + b1396 DVU0601 0.77 -0.7 672,522 - +0.0
Or see this region's nucleotide sequence