Experiment: MoYLS4 with Spectinomycin 0.0125mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cas3 and DVUA0130 are separated by 13 nucleotides DVUA0130 and DVUA0131 overlap by 4 nucleotides
DVUA0129: cas3 - CRISPR-associated helicase Cas3 domain protein (TIGR), at 168,254 to 170,362
cas3
DVUA0130: DVUA0130 - CRISPR-associated protein, CT1134 family (TIGR), at 170,376 to 171,059
DVUA0130
DVUA0131: DVUA0131 - CRISPR-associated protein, CT1133 family (TIGR), at 171,056 to 172,894
DVUA0131
Position (kb) 170
171
172 Strain fitness (log2 ratio) -2
-1
0
1 at 169.392 kb on + strand, within cas3 at 169.392 kb on - strand, within cas3 at 169.405 kb on - strand, within cas3 at 169.438 kb on + strand, within cas3 at 169.438 kb on + strand, within cas3 at 169.446 kb on - strand, within cas3 at 169.480 kb on - strand, within cas3 at 169.486 kb on + strand, within cas3 at 169.532 kb on - strand, within cas3 at 169.661 kb on - strand, within cas3 at 169.747 kb on - strand, within cas3 at 169.795 kb on - strand, within cas3 at 169.820 kb on - strand, within cas3 at 169.854 kb on - strand, within cas3 at 169.879 kb on - strand, within cas3 at 169.879 kb on - strand, within cas3 at 169.879 kb on - strand, within cas3 at 169.937 kb on + strand, within cas3 at 169.966 kb on + strand, within cas3 at 169.966 kb on + strand, within cas3 at 169.966 kb on + strand, within cas3 at 169.974 kb on - strand, within cas3 at 170.115 kb on + strand, within cas3 at 170.123 kb on + strand, within cas3 at 170.166 kb on + strand at 170.166 kb on + strand at 170.166 kb on + strand at 170.174 kb on - strand at 170.174 kb on - strand at 170.179 kb on - strand at 170.181 kb on + strand at 170.268 kb on + strand at 170.304 kb on + strand at 170.333 kb on - strand at 170.404 kb on + strand at 170.425 kb on - strand at 170.489 kb on - strand, within DVUA0130 at 170.509 kb on + strand, within DVUA0130 at 170.510 kb on + strand, within DVUA0130 at 170.517 kb on - strand, within DVUA0130 at 170.560 kb on + strand, within DVUA0130 at 170.565 kb on - strand, within DVUA0130 at 170.702 kb on - strand, within DVUA0130 at 170.763 kb on + strand, within DVUA0130 at 170.796 kb on + strand, within DVUA0130 at 170.959 kb on - strand, within DVUA0130 at 171.135 kb on + strand at 171.153 kb on - strand at 171.355 kb on + strand, within DVUA0131 at 171.363 kb on - strand, within DVUA0131 at 171.379 kb on + strand, within DVUA0131 at 171.387 kb on - strand, within DVUA0131 at 171.404 kb on + strand, within DVUA0131 at 171.532 kb on + strand, within DVUA0131 at 171.585 kb on + strand, within DVUA0131 at 171.607 kb on - strand, within DVUA0131 at 171.607 kb on - strand, within DVUA0131 at 171.612 kb on + strand, within DVUA0131 at 171.655 kb on + strand, within DVUA0131 at 171.663 kb on - strand, within DVUA0131 at 171.712 kb on - strand, within DVUA0131 at 171.789 kb on + strand, within DVUA0131 at 171.800 kb on + strand, within DVUA0131 at 171.800 kb on + strand, within DVUA0131 at 171.838 kb on + strand, within DVUA0131 at 171.865 kb on + strand, within DVUA0131 at 171.873 kb on - strand, within DVUA0131 at 171.888 kb on - strand, within DVUA0131 at 171.893 kb on + strand, within DVUA0131 at 171.930 kb on - strand, within DVUA0131 at 171.991 kb on + strand, within DVUA0131
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.0125mg/mL remove 169,392 + cas3 DVUA0129 0.54 +0.6 169,392 - cas3 DVUA0129 0.54 +0.1 169,405 - cas3 DVUA0129 0.55 -0.2 169,438 + cas3 DVUA0129 0.56 +0.2 169,438 + cas3 DVUA0129 0.56 +0.1 169,446 - cas3 DVUA0129 0.57 +0.9 169,480 - cas3 DVUA0129 0.58 -1.3 169,486 + cas3 DVUA0129 0.58 -0.0 169,532 - cas3 DVUA0129 0.61 +0.2 169,661 - cas3 DVUA0129 0.67 +0.3 169,747 - cas3 DVUA0129 0.71 -0.2 169,795 - cas3 DVUA0129 0.73 +0.1 169,820 - cas3 DVUA0129 0.74 +0.4 169,854 - cas3 DVUA0129 0.76 -0.4 169,879 - cas3 DVUA0129 0.77 +0.5 169,879 - cas3 DVUA0129 0.77 -0.2 169,879 - cas3 DVUA0129 0.77 +0.2 169,937 + cas3 DVUA0129 0.80 +0.2 169,966 + cas3 DVUA0129 0.81 -1.7 169,966 + cas3 DVUA0129 0.81 -0.3 169,966 + cas3 DVUA0129 0.81 -0.1 169,974 - cas3 DVUA0129 0.82 +0.0 170,115 + cas3 DVUA0129 0.88 -0.4 170,123 + cas3 DVUA0129 0.89 +1.0 170,166 + -0.2 170,166 + -0.4 170,166 + +0.3 170,174 - -0.2 170,174 - +0.1 170,179 - +0.1 170,181 + -0.3 170,268 + +0.1 170,304 + -0.5 170,333 - -0.3 170,404 + -0.0 170,425 - -0.3 170,489 - DVUA0130 0.17 -0.2 170,509 + DVUA0130 0.19 -0.0 170,510 + DVUA0130 0.20 -1.0 170,517 - DVUA0130 0.21 -0.8 170,560 + DVUA0130 0.27 -1.0 170,565 - DVUA0130 0.28 -0.6 170,702 - DVUA0130 0.48 -1.0 170,763 + DVUA0130 0.57 +0.7 170,796 + DVUA0130 0.61 -0.3 170,959 - DVUA0130 0.85 -0.5 171,135 + -0.0 171,153 - +0.6 171,355 + DVUA0131 0.16 -0.6 171,363 - DVUA0131 0.17 -0.0 171,379 + DVUA0131 0.18 +0.1 171,387 - DVUA0131 0.18 -0.6 171,404 + DVUA0131 0.19 +0.2 171,532 + DVUA0131 0.26 +0.3 171,585 + DVUA0131 0.29 +0.1 171,607 - DVUA0131 0.30 -0.3 171,607 - DVUA0131 0.30 +0.1 171,612 + DVUA0131 0.30 -0.1 171,655 + DVUA0131 0.33 +0.7 171,663 - DVUA0131 0.33 +0.1 171,712 - DVUA0131 0.36 -0.4 171,789 + DVUA0131 0.40 +1.0 171,800 + DVUA0131 0.40 +0.1 171,800 + DVUA0131 0.40 -2.1 171,838 + DVUA0131 0.43 -0.4 171,865 + DVUA0131 0.44 -0.4 171,873 - DVUA0131 0.44 -0.1 171,888 - DVUA0131 0.45 +0.4 171,893 + DVUA0131 0.46 +0.1 171,930 - DVUA0131 0.48 +0.4 171,991 + DVUA0131 0.51 -0.0
Or see this region's nucleotide sequence