Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20775

Experiment: JK38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20765 and ECOLIN_RS20770 are separated by 291 nucleotidesECOLIN_RS20770 and ECOLIN_RS20775 overlap by 4 nucleotidesECOLIN_RS20775 and ECOLIN_RS20780 are separated by 257 nucleotidesECOLIN_RS20780 and ECOLIN_RS20785 are separated by 54 nucleotides ECOLIN_RS20765: ECOLIN_RS20765 - DNA damage-inducible protein D, at 4,182,093 to 4,182,917 _RS20765 ECOLIN_RS20770: ECOLIN_RS20770 - trimeric intracellular cation channel family protein, at 4,183,209 to 4,183,826 _RS20770 ECOLIN_RS20775: ECOLIN_RS20775 - NAD-dependent DNA ligase LigB, at 4,183,823 to 4,185,505 _RS20775 ECOLIN_RS20780: ECOLIN_RS20780 - guanylate kinase, at 4,185,763 to 4,186,386 _RS20780 ECOLIN_RS20785: ECOLIN_RS20785 - DNA-directed RNA polymerase subunit omega, at 4,186,441 to 4,186,716 _RS20785 Position (kb) 4183 4184 4185 4186Strain fitness (log2 ratio) -1 0 1at 4183.083 kb on + strandat 4183.083 kb on + strandat 4183.083 kb on + strandat 4183.083 kb on + strandat 4183.084 kb on - strandat 4183.084 kb on - strandat 4183.084 kb on - strandat 4183.084 kb on - strandat 4183.084 kb on - strandat 4183.084 kb on - strandat 4183.084 kb on - strandat 4183.084 kb on - strandat 4183.217 kb on - strandat 4183.304 kb on - strand, within ECOLIN_RS20770at 4183.307 kb on + strand, within ECOLIN_RS20770at 4183.308 kb on - strand, within ECOLIN_RS20770at 4183.398 kb on + strand, within ECOLIN_RS20770at 4183.398 kb on + strand, within ECOLIN_RS20770at 4183.407 kb on - strand, within ECOLIN_RS20770at 4183.436 kb on + strand, within ECOLIN_RS20770at 4183.438 kb on + strand, within ECOLIN_RS20770at 4183.438 kb on + strand, within ECOLIN_RS20770at 4183.439 kb on - strand, within ECOLIN_RS20770at 4183.439 kb on - strand, within ECOLIN_RS20770at 4183.439 kb on - strand, within ECOLIN_RS20770at 4183.439 kb on - strand, within ECOLIN_RS20770at 4183.439 kb on - strand, within ECOLIN_RS20770at 4183.458 kb on + strand, within ECOLIN_RS20770at 4183.459 kb on - strand, within ECOLIN_RS20770at 4183.511 kb on + strand, within ECOLIN_RS20770at 4183.556 kb on + strand, within ECOLIN_RS20770at 4183.557 kb on - strand, within ECOLIN_RS20770at 4183.653 kb on + strand, within ECOLIN_RS20770at 4183.653 kb on + strand, within ECOLIN_RS20770at 4183.654 kb on - strand, within ECOLIN_RS20770at 4183.686 kb on + strand, within ECOLIN_RS20770at 4183.686 kb on + strand, within ECOLIN_RS20770at 4183.704 kb on + strand, within ECOLIN_RS20770at 4183.705 kb on - strand, within ECOLIN_RS20770at 4183.807 kb on - strandat 4183.903 kb on + strandat 4184.129 kb on + strand, within ECOLIN_RS20775at 4184.130 kb on - strand, within ECOLIN_RS20775at 4184.147 kb on + strand, within ECOLIN_RS20775at 4184.147 kb on + strand, within ECOLIN_RS20775at 4184.164 kb on + strand, within ECOLIN_RS20775at 4184.255 kb on + strand, within ECOLIN_RS20775at 4184.256 kb on - strand, within ECOLIN_RS20775at 4184.298 kb on + strand, within ECOLIN_RS20775at 4184.583 kb on - strand, within ECOLIN_RS20775at 4184.717 kb on + strand, within ECOLIN_RS20775at 4184.758 kb on - strand, within ECOLIN_RS20775at 4184.964 kb on - strand, within ECOLIN_RS20775at 4184.990 kb on + strand, within ECOLIN_RS20775at 4185.179 kb on + strand, within ECOLIN_RS20775at 4185.179 kb on + strand, within ECOLIN_RS20775at 4185.180 kb on - strand, within ECOLIN_RS20775at 4185.180 kb on - strand, within ECOLIN_RS20775at 4185.314 kb on + strand, within ECOLIN_RS20775at 4185.383 kb on + strandat 4185.384 kb on - strandat 4185.475 kb on + strandat 4185.479 kb on + strandat 4185.479 kb on + strandat 4185.479 kb on + strandat 4185.480 kb on - strandat 4185.483 kb on - strandat 4185.483 kb on - strandat 4185.486 kb on - strandat 4185.497 kb on + strandat 4185.498 kb on - strandat 4185.521 kb on - strandat 4185.521 kb on - strandat 4185.525 kb on - strandat 4185.525 kb on - strandat 4185.536 kb on + strandat 4185.538 kb on + strandat 4185.538 kb on + strandat 4185.538 kb on + strandat 4185.539 kb on - strandat 4185.539 kb on - strandat 4185.539 kb on - strandat 4185.609 kb on - strandat 4185.688 kb on - strandat 4185.726 kb on + strandat 4185.726 kb on + strandat 4185.749 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK38
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4,183,083 + -0.5
4,183,083 + -0.4
4,183,083 + +0.5
4,183,083 + -0.2
4,183,084 - +0.0
4,183,084 - -0.2
4,183,084 - +0.1
4,183,084 - +0.0
4,183,084 - +0.3
4,183,084 - +0.1
4,183,084 - -0.0
4,183,084 - +0.4
4,183,217 - +0.0
4,183,304 - ECOLIN_RS20770 0.15 -0.0
4,183,307 + ECOLIN_RS20770 0.16 -0.4
4,183,308 - ECOLIN_RS20770 0.16 +0.0
4,183,398 + ECOLIN_RS20770 0.31 +0.1
4,183,398 + ECOLIN_RS20770 0.31 -0.4
4,183,407 - ECOLIN_RS20770 0.32 -0.1
4,183,436 + ECOLIN_RS20770 0.37 -0.1
4,183,438 + ECOLIN_RS20770 0.37 -0.0
4,183,438 + ECOLIN_RS20770 0.37 +0.1
4,183,439 - ECOLIN_RS20770 0.37 -0.4
4,183,439 - ECOLIN_RS20770 0.37 -0.2
4,183,439 - ECOLIN_RS20770 0.37 -0.1
4,183,439 - ECOLIN_RS20770 0.37 -0.2
4,183,439 - ECOLIN_RS20770 0.37 +0.9
4,183,458 + ECOLIN_RS20770 0.40 -0.2
4,183,459 - ECOLIN_RS20770 0.40 -0.6
4,183,511 + ECOLIN_RS20770 0.49 -0.3
4,183,556 + ECOLIN_RS20770 0.56 +0.1
4,183,557 - ECOLIN_RS20770 0.56 -0.3
4,183,653 + ECOLIN_RS20770 0.72 -0.4
4,183,653 + ECOLIN_RS20770 0.72 +0.6
4,183,654 - ECOLIN_RS20770 0.72 -0.2
4,183,686 + ECOLIN_RS20770 0.77 -0.4
4,183,686 + ECOLIN_RS20770 0.77 +0.2
4,183,704 + ECOLIN_RS20770 0.80 +0.2
4,183,705 - ECOLIN_RS20770 0.80 -1.2
4,183,807 - -0.4
4,183,903 + -0.1
4,184,129 + ECOLIN_RS20775 0.18 -0.1
4,184,130 - ECOLIN_RS20775 0.18 -0.1
4,184,147 + ECOLIN_RS20775 0.19 +0.9
4,184,147 + ECOLIN_RS20775 0.19 -0.0
4,184,164 + ECOLIN_RS20775 0.20 +0.2
4,184,255 + ECOLIN_RS20775 0.26 -0.2
4,184,256 - ECOLIN_RS20775 0.26 +0.5
4,184,298 + ECOLIN_RS20775 0.28 -0.2
4,184,583 - ECOLIN_RS20775 0.45 +0.3
4,184,717 + ECOLIN_RS20775 0.53 -0.5
4,184,758 - ECOLIN_RS20775 0.56 +0.6
4,184,964 - ECOLIN_RS20775 0.68 -0.1
4,184,990 + ECOLIN_RS20775 0.69 -1.1
4,185,179 + ECOLIN_RS20775 0.81 -0.1
4,185,179 + ECOLIN_RS20775 0.81 -0.3
4,185,180 - ECOLIN_RS20775 0.81 -0.1
4,185,180 - ECOLIN_RS20775 0.81 -0.1
4,185,314 + ECOLIN_RS20775 0.89 -0.1
4,185,383 + -0.0
4,185,384 - +0.2
4,185,475 + +0.2
4,185,479 + +0.5
4,185,479 + -0.2
4,185,479 + -0.1
4,185,480 - +0.3
4,185,483 - -0.0
4,185,483 - -0.0
4,185,486 - -0.3
4,185,497 + +0.5
4,185,498 - +0.0
4,185,521 - -0.1
4,185,521 - -0.7
4,185,525 - -0.0
4,185,525 - -0.1
4,185,536 + +0.1
4,185,538 + +0.0
4,185,538 + -0.2
4,185,538 + -0.9
4,185,539 - -0.3
4,185,539 - +0.4
4,185,539 - -0.3
4,185,609 - +0.9
4,185,688 - +0.1
4,185,726 + +0.3
4,185,726 + +0.5
4,185,749 + -0.7

Or see this region's nucleotide sequence