Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS17000

Experiment: JK38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS32100 and ECOLIN_RS17000 are separated by 810 nucleotidesECOLIN_RS17000 and ECOLIN_RS17005 are separated by 71 nucleotides ECOLIN_RS32100: ECOLIN_RS32100 - IS110 family transposase, at 3,419,790 to 3,419,960 _RS32100 ECOLIN_RS17000: ECOLIN_RS17000 - KpsF/GutQ family sugar-phosphate isomerase, at 3,420,771 to 3,421,754 _RS17000 ECOLIN_RS17005: ECOLIN_RS17005 - capsule polysaccharide export inner-membrane protein KpsE, at 3,421,826 to 3,422,974 _RS17005 Position (kb) 3420 3421 3422Strain fitness (log2 ratio) -8 -7 -6 -5 -4 -3 -2 -1 0 1at 3419.853 kb on + strand, within ECOLIN_RS32100at 3419.907 kb on - strand, within ECOLIN_RS32100at 3419.907 kb on - strand, within ECOLIN_RS32100at 3420.024 kb on + strandat 3420.115 kb on - strandat 3420.617 kb on + strandat 3420.641 kb on + strandat 3420.748 kb on + strandat 3420.935 kb on + strand, within ECOLIN_RS17000at 3421.384 kb on + strand, within ECOLIN_RS17000at 3421.752 kb on + strandat 3421.756 kb on + strandat 3421.756 kb on + strandat 3421.756 kb on + strandat 3421.756 kb on + strandat 3421.756 kb on + strandat 3421.757 kb on - strandat 3421.823 kb on + strandat 3421.982 kb on - strand, within ECOLIN_RS17005at 3422.022 kb on + strand, within ECOLIN_RS17005at 3422.261 kb on + strand, within ECOLIN_RS17005at 3422.261 kb on + strand, within ECOLIN_RS17005at 3422.536 kb on + strand, within ECOLIN_RS17005at 3422.591 kb on + strand, within ECOLIN_RS17005

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Per-strain Table

Position Strand Gene LocusTag Fraction JK38
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3,419,853 + ECOLIN_RS32100 0.37 -1.1
3,419,907 - ECOLIN_RS32100 0.68 +0.5
3,419,907 - ECOLIN_RS32100 0.68 +0.4
3,420,024 + -0.6
3,420,115 - +0.3
3,420,617 + -1.3
3,420,641 + -1.5
3,420,748 + -6.2
3,420,935 + ECOLIN_RS17000 0.17 -4.2
3,421,384 + ECOLIN_RS17000 0.62 -2.2
3,421,752 + -0.5
3,421,756 + -1.8
3,421,756 + -2.0
3,421,756 + -2.2
3,421,756 + -3.4
3,421,756 + -2.6
3,421,757 - -7.8
3,421,823 + -4.1
3,421,982 - ECOLIN_RS17005 0.14 -4.1
3,422,022 + ECOLIN_RS17005 0.17 -8.1
3,422,261 + ECOLIN_RS17005 0.38 -3.9
3,422,261 + ECOLIN_RS17005 0.38 -4.4
3,422,536 + ECOLIN_RS17005 0.62 +0.1
3,422,591 + ECOLIN_RS17005 0.67 -4.1

Or see this region's nucleotide sequence