Experiment: JK38
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS16165 and ECOLIN_RS16170 are separated by 117 nucleotides ECOLIN_RS16170 and ECOLIN_RS16175 are separated by 23 nucleotides ECOLIN_RS16175 and ECOLIN_RS31590 are separated by 290 nucleotides ECOLIN_RS31590 and ECOLIN_RS16180 are separated by 73 nucleotides
ECOLIN_RS16165: ECOLIN_RS16165 - aminomethyl-transferring glycine dehydrogenase, at 3,277,752 to 3,280,625
_RS16165
ECOLIN_RS16170: ECOLIN_RS16170 - glycine cleavage system protein GcvH, at 3,280,743 to 3,281,132
_RS16170
ECOLIN_RS16175: ECOLIN_RS16175 - glycine cleavage system aminomethyltransferase GcvT, at 3,281,156 to 3,282,250
_RS16175
ECOLIN_RS31590: ECOLIN_RS31590 - hypothetical protein, at 3,282,541 to 3,282,624
_RS31590
ECOLIN_RS16180: ECOLIN_RS16180 - FAD-dependent 2-octaprenylphenol hydroxylase, at 3,282,698 to 3,283,900
_RS16180
Position (kb)
3281
3282
3283 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3280.301 kb on - strand, within ECOLIN_RS16165 at 3280.326 kb on - strand, within ECOLIN_RS16165 at 3280.326 kb on - strand, within ECOLIN_RS16165 at 3280.375 kb on + strand at 3280.583 kb on + strand at 3280.612 kb on - strand at 3280.756 kb on - strand at 3280.756 kb on - strand at 3280.844 kb on - strand, within ECOLIN_RS16170 at 3281.060 kb on - strand, within ECOLIN_RS16170 at 3281.079 kb on - strand, within ECOLIN_RS16170 at 3281.105 kb on - strand at 3281.122 kb on - strand at 3281.204 kb on - strand at 3281.252 kb on - strand at 3281.281 kb on + strand, within ECOLIN_RS16175 at 3281.281 kb on + strand, within ECOLIN_RS16175 at 3281.282 kb on - strand, within ECOLIN_RS16175 at 3281.282 kb on - strand, within ECOLIN_RS16175 at 3281.282 kb on - strand, within ECOLIN_RS16175 at 3281.282 kb on - strand, within ECOLIN_RS16175 at 3281.282 kb on - strand, within ECOLIN_RS16175 at 3281.306 kb on - strand, within ECOLIN_RS16175 at 3281.544 kb on + strand, within ECOLIN_RS16175 at 3282.098 kb on - strand, within ECOLIN_RS16175 at 3282.127 kb on + strand, within ECOLIN_RS16175 at 3282.696 kb on - strand at 3282.696 kb on - strand at 3282.700 kb on - strand at 3282.854 kb on - strand, within ECOLIN_RS16180 at 3282.995 kb on + strand, within ECOLIN_RS16180 at 3283.125 kb on - strand, within ECOLIN_RS16180
Per-strain Table
Position Strand Gene LocusTag Fraction JK38 remove 3,280,301 - ECOLIN_RS16165 0.89 +0.2 3,280,326 - ECOLIN_RS16165 0.90 +0.4 3,280,326 - ECOLIN_RS16165 0.90 +0.3 3,280,375 + -1.5 3,280,583 + -3.8 3,280,612 - +1.5 3,280,756 - +0.3 3,280,756 - -0.3 3,280,844 - ECOLIN_RS16170 0.26 -0.2 3,281,060 - ECOLIN_RS16170 0.81 +0.1 3,281,079 - ECOLIN_RS16170 0.86 +0.2 3,281,105 - -0.4 3,281,122 - +0.3 3,281,204 - -0.0 3,281,252 - -1.0 3,281,281 + ECOLIN_RS16175 0.11 -0.2 3,281,281 + ECOLIN_RS16175 0.11 -0.7 3,281,282 - ECOLIN_RS16175 0.12 -0.1 3,281,282 - ECOLIN_RS16175 0.12 -0.6 3,281,282 - ECOLIN_RS16175 0.12 -0.6 3,281,282 - ECOLIN_RS16175 0.12 -0.0 3,281,282 - ECOLIN_RS16175 0.12 +0.1 3,281,306 - ECOLIN_RS16175 0.14 +0.3 3,281,544 + ECOLIN_RS16175 0.35 -1.1 3,282,098 - ECOLIN_RS16175 0.86 -1.8 3,282,127 + ECOLIN_RS16175 0.89 -0.6 3,282,696 - -0.4 3,282,696 - -0.3 3,282,700 - +0.0 3,282,854 - ECOLIN_RS16180 0.13 -0.7 3,282,995 + ECOLIN_RS16180 0.25 -3.6 3,283,125 - ECOLIN_RS16180 0.35 -3.5
Or see this region's nucleotide sequence