Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS10045

Experiment: JK38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS10040 and ECOLIN_RS10045 are separated by 204 nucleotidesECOLIN_RS10045 and ECOLIN_RS10050 are separated by 39 nucleotidesECOLIN_RS10050 and ECOLIN_RS10055 are separated by 57 nucleotides ECOLIN_RS10040: ECOLIN_RS10040 - long-chain-fatty-acid--CoA ligase FadD, at 1,972,046 to 1,973,731 _RS10040 ECOLIN_RS10045: ECOLIN_RS10045 - Slp family lipoprotein YeaY, at 1,973,936 to 1,974,517 _RS10045 ECOLIN_RS10050: ECOLIN_RS10050 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB, at 1,974,557 to 1,975,252 _RS10050 ECOLIN_RS10055: ECOLIN_RS10055 - ATP-dependent DNA helicase, at 1,975,310 to 1,977,220 _RS10055 Position (kb) 1973 1974 1975Strain fitness (log2 ratio) -2 -1 0 1at 1973.008 kb on - strand, within ECOLIN_RS10040at 1974.004 kb on - strand, within ECOLIN_RS10045at 1974.445 kb on + strand, within ECOLIN_RS10045at 1975.267 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK38
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1,973,008 - ECOLIN_RS10040 0.57 -0.2
1,974,004 - ECOLIN_RS10045 0.12 -0.4
1,974,445 + ECOLIN_RS10045 0.87 -2.3
1,975,267 - -2.4

Or see this region's nucleotide sequence