Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS15365

Experiment: JK38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS15355 and ECOLIN_RS15360 overlap by 1 nucleotidesECOLIN_RS15360 and ECOLIN_RS15365 are separated by 18 nucleotidesECOLIN_RS15365 and ECOLIN_RS15370 are separated by 192 nucleotidesECOLIN_RS15370 and ECOLIN_RS15375 are separated by 51 nucleotides ECOLIN_RS15355: ECOLIN_RS15355 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 3,089,808 to 3,090,287 _RS15355 ECOLIN_RS15360: ECOLIN_RS15360 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 3,090,287 to 3,090,997 _RS15360 ECOLIN_RS15365: ECOLIN_RS15365 - cell division protein FtsB, at 3,091,016 to 3,091,327 _RS15365 ECOLIN_RS15370: ECOLIN_RS15370 - DUF3561 family protein, at 3,091,520 to 3,091,843 _RS15370 ECOLIN_RS15375: ECOLIN_RS15375 - adenylyl-sulfate kinase, at 3,091,895 to 3,092,500 _RS15375 Position (kb) 3091 3092Strain fitness (log2 ratio) -2 -1 0 1at 3090.289 kb on - strandat 3090.293 kb on - strandat 3091.379 kb on - strandat 3091.379 kb on - strandat 3091.438 kb on + strandat 3091.439 kb on - strandat 3091.439 kb on - strandat 3091.442 kb on + strandat 3091.443 kb on - strandat 3091.443 kb on - strandat 3091.443 kb on - strandat 3091.464 kb on + strandat 3091.493 kb on - strandat 3091.503 kb on + strandat 3091.537 kb on + strandat 3091.585 kb on + strand, within ECOLIN_RS15370at 3091.585 kb on + strand, within ECOLIN_RS15370at 3091.585 kb on + strand, within ECOLIN_RS15370at 3091.677 kb on + strand, within ECOLIN_RS15370at 3091.677 kb on + strand, within ECOLIN_RS15370at 3091.678 kb on - strand, within ECOLIN_RS15370at 3092.057 kb on - strand, within ECOLIN_RS15375at 3092.180 kb on - strand, within ECOLIN_RS15375at 3092.326 kb on + strand, within ECOLIN_RS15375

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Per-strain Table

Position Strand Gene LocusTag Fraction JK38
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3,090,289 - -2.3
3,090,293 - -0.5
3,091,379 - -0.5
3,091,379 - -0.5
3,091,438 + +0.1
3,091,439 - -0.0
3,091,439 - -0.8
3,091,442 + +0.1
3,091,443 - -0.4
3,091,443 - +0.4
3,091,443 - -0.3
3,091,464 + +1.3
3,091,493 - -0.1
3,091,503 + +0.1
3,091,537 + -1.9
3,091,585 + ECOLIN_RS15370 0.20 -0.0
3,091,585 + ECOLIN_RS15370 0.20 -0.5
3,091,585 + ECOLIN_RS15370 0.20 -0.4
3,091,677 + ECOLIN_RS15370 0.48 +1.1
3,091,677 + ECOLIN_RS15370 0.48 -1.4
3,091,678 - ECOLIN_RS15370 0.49 -0.4
3,092,057 - ECOLIN_RS15375 0.27 -1.0
3,092,180 - ECOLIN_RS15375 0.47 +0.0
3,092,326 + ECOLIN_RS15375 0.71 +0.8

Or see this region's nucleotide sequence