Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS14220

Experiment: JK38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS14210 and ECOLIN_RS32185 are separated by 181 nucleotidesECOLIN_RS32185 and ECOLIN_RS14220 are separated by 17 nucleotidesECOLIN_RS14220 and ECOLIN_RS14225 are separated by 310 nucleotidesECOLIN_RS14225 and ECOLIN_RS14230 are separated by 15 nucleotides ECOLIN_RS14210: ECOLIN_RS14210 - cyclic-guanylate-specific phosphodiesterase PdeF, at 2,855,724 to 2,857,967 _RS14210 ECOLIN_RS32185: ECOLIN_RS32185 - hypothetical protein, at 2,858,149 to 2,858,301 _RS32185 ECOLIN_RS14220: ECOLIN_RS14220 - YfgG family protein, at 2,858,319 to 2,858,510 _RS14220 ECOLIN_RS14225: ECOLIN_RS14225 - glycine zipper 2TM domain-containing protein, at 2,858,821 to 2,859,339 _RS14225 ECOLIN_RS14230: ECOLIN_RS14230 - DUF5384 family protein, at 2,859,355 to 2,859,894 _RS14230 Position (kb) 2858 2859Strain fitness (log2 ratio) -1 0 1at 2858.397 kb on - strand, within ECOLIN_RS14220at 2859.220 kb on + strand, within ECOLIN_RS14225

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Per-strain Table

Position Strand Gene LocusTag Fraction JK38
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2,858,397 - ECOLIN_RS14220 0.41 -0.1
2,859,220 + ECOLIN_RS14225 0.77 -0.9

Or see this region's nucleotide sequence