Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS33120

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS24695 and ECOLIN_RS33120 are separated by 153 nucleotidesECOLIN_RS33120 and ECOLIN_RS24700 are separated by 62 nucleotides ECOLIN_RS24695: ECOLIN_RS24695 - leucyl aminopeptidase, at 4,968,330 to 4,969,841 _RS24695 ECOLIN_RS33120: ECOLIN_RS33120 - protein YtgA, at 4,969,995 to 4,970,045 _RS33120 ECOLIN_RS24700: ECOLIN_RS24700 - LPS export ABC transporter permease LptF, at 4,970,108 to 4,971,208 _RS24700 Position (kb) 4969 4970 4971Strain fitness (log2 ratio) -1 0 1at 4969.135 kb on + strand, within ECOLIN_RS24695at 4969.135 kb on + strand, within ECOLIN_RS24695at 4969.201 kb on + strand, within ECOLIN_RS24695at 4969.238 kb on - strand, within ECOLIN_RS24695at 4969.430 kb on - strand, within ECOLIN_RS24695at 4969.491 kb on - strand, within ECOLIN_RS24695at 4969.547 kb on + strand, within ECOLIN_RS24695at 4969.548 kb on - strand, within ECOLIN_RS24695at 4969.576 kb on + strand, within ECOLIN_RS24695at 4969.717 kb on + strandat 4969.832 kb on + strandat 4970.001 kb on - strand, within ECOLIN_RS33120at 4970.011 kb on + strand, within ECOLIN_RS33120

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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4,969,135 + ECOLIN_RS24695 0.53 +0.0
4,969,135 + ECOLIN_RS24695 0.53 +0.1
4,969,201 + ECOLIN_RS24695 0.58 +0.1
4,969,238 - ECOLIN_RS24695 0.60 +1.0
4,969,430 - ECOLIN_RS24695 0.73 -0.1
4,969,491 - ECOLIN_RS24695 0.77 -0.1
4,969,547 + ECOLIN_RS24695 0.80 -0.2
4,969,548 - ECOLIN_RS24695 0.81 +0.1
4,969,576 + ECOLIN_RS24695 0.82 -0.4
4,969,717 + -0.4
4,969,832 + +0.6
4,970,001 - ECOLIN_RS33120 0.12 -0.7
4,970,011 + ECOLIN_RS33120 0.31 -0.4

Or see this region's nucleotide sequence