Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20685

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20680 and ECOLIN_RS20685 are separated by 16 nucleotidesECOLIN_RS20685 and ECOLIN_RS20690 are separated by 15 nucleotidesECOLIN_RS20690 and ECOLIN_RS20695 overlap by 8 nucleotides ECOLIN_RS20680: ECOLIN_RS20680 - glycosyltransferase, at 4,167,445 to 4,168,440 _RS20680 ECOLIN_RS20685: ECOLIN_RS20685 - lipopolysaccharide 3-alpha-galactosyltransferase, at 4,168,457 to 4,169,473 _RS20685 ECOLIN_RS20690: ECOLIN_RS20690 - lipopolysaccharide core heptose(I) kinase RfaP, at 4,169,489 to 4,170,286 _RS20690 ECOLIN_RS20695: ECOLIN_RS20695 - glycosyltransferase family 4 protein, at 4,170,279 to 4,171,403 _RS20695 Position (kb) 4168 4169 4170Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4167.741 kb on + strand, within ECOLIN_RS20680at 4167.903 kb on - strand, within ECOLIN_RS20680at 4167.975 kb on + strand, within ECOLIN_RS20680at 4168.015 kb on - strand, within ECOLIN_RS20680at 4168.021 kb on - strand, within ECOLIN_RS20680at 4168.128 kb on - strand, within ECOLIN_RS20680at 4168.128 kb on - strand, within ECOLIN_RS20680at 4168.338 kb on - strand, within ECOLIN_RS20680at 4168.489 kb on + strandat 4169.040 kb on - strand, within ECOLIN_RS20685at 4169.040 kb on - strand, within ECOLIN_RS20685at 4169.126 kb on + strand, within ECOLIN_RS20685at 4169.140 kb on - strand, within ECOLIN_RS20685at 4169.150 kb on - strand, within ECOLIN_RS20685at 4169.219 kb on - strand, within ECOLIN_RS20685at 4169.219 kb on - strand, within ECOLIN_RS20685at 4169.223 kb on + strand, within ECOLIN_RS20685at 4169.311 kb on + strand, within ECOLIN_RS20685at 4169.312 kb on - strand, within ECOLIN_RS20685at 4169.456 kb on - strandat 4169.733 kb on - strand, within ECOLIN_RS20690at 4169.794 kb on + strand, within ECOLIN_RS20690at 4169.796 kb on + strand, within ECOLIN_RS20690at 4169.860 kb on + strand, within ECOLIN_RS20690at 4169.861 kb on - strand, within ECOLIN_RS20690at 4169.867 kb on - strand, within ECOLIN_RS20690at 4170.010 kb on - strand, within ECOLIN_RS20690at 4170.013 kb on + strand, within ECOLIN_RS20690at 4170.013 kb on + strand, within ECOLIN_RS20690at 4170.013 kb on + strand, within ECOLIN_RS20690at 4170.014 kb on - strand, within ECOLIN_RS20690at 4170.014 kb on - strand, within ECOLIN_RS20690at 4170.014 kb on - strand, within ECOLIN_RS20690at 4170.014 kb on - strand, within ECOLIN_RS20690at 4170.108 kb on - strand, within ECOLIN_RS20690at 4170.275 kb on - strandat 4170.332 kb on - strandat 4170.333 kb on + strandat 4170.334 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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4,167,741 + ECOLIN_RS20680 0.30 -3.2
4,167,903 - ECOLIN_RS20680 0.46 -1.6
4,167,975 + ECOLIN_RS20680 0.53 -3.5
4,168,015 - ECOLIN_RS20680 0.57 -1.2
4,168,021 - ECOLIN_RS20680 0.58 +0.0
4,168,128 - ECOLIN_RS20680 0.69 -0.8
4,168,128 - ECOLIN_RS20680 0.69 -0.9
4,168,338 - ECOLIN_RS20680 0.90 -1.0
4,168,489 + -2.5
4,169,040 - ECOLIN_RS20685 0.57 -1.5
4,169,040 - ECOLIN_RS20685 0.57 -2.3
4,169,126 + ECOLIN_RS20685 0.66 -1.3
4,169,140 - ECOLIN_RS20685 0.67 -1.3
4,169,150 - ECOLIN_RS20685 0.68 -0.7
4,169,219 - ECOLIN_RS20685 0.75 -2.3
4,169,219 - ECOLIN_RS20685 0.75 -2.1
4,169,223 + ECOLIN_RS20685 0.75 -2.1
4,169,311 + ECOLIN_RS20685 0.84 -1.9
4,169,312 - ECOLIN_RS20685 0.84 -2.2
4,169,456 - -0.4
4,169,733 - ECOLIN_RS20690 0.31 -1.9
4,169,794 + ECOLIN_RS20690 0.38 -3.2
4,169,796 + ECOLIN_RS20690 0.38 +1.1
4,169,860 + ECOLIN_RS20690 0.46 -3.5
4,169,861 - ECOLIN_RS20690 0.47 -1.9
4,169,867 - ECOLIN_RS20690 0.47 -3.6
4,170,010 - ECOLIN_RS20690 0.65 -2.3
4,170,013 + ECOLIN_RS20690 0.66 -2.6
4,170,013 + ECOLIN_RS20690 0.66 -3.2
4,170,013 + ECOLIN_RS20690 0.66 -3.8
4,170,014 - ECOLIN_RS20690 0.66 -1.5
4,170,014 - ECOLIN_RS20690 0.66 -1.5
4,170,014 - ECOLIN_RS20690 0.66 -4.8
4,170,014 - ECOLIN_RS20690 0.66 -3.3
4,170,108 - ECOLIN_RS20690 0.78 -2.6
4,170,275 - -4.2
4,170,332 - -2.9
4,170,333 + -2.6
4,170,334 - +0.3

Or see this region's nucleotide sequence