Experiment: Bas10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS17915 and ECOLIN_RS17920 are separated by 71 nucleotides ECOLIN_RS17920 and ECOLIN_RS17925 are separated by 13 nucleotides ECOLIN_RS17925 and ECOLIN_RS17930 are separated by 33 nucleotides
ECOLIN_RS17915: ECOLIN_RS17915 - L-serine ammonia-lyase, at 3,611,433 to 3,612,797
_RS17915
ECOLIN_RS17920: ECOLIN_RS17920 - enamine/imine deaminase, at 3,612,869 to 3,613,258
_RS17920
ECOLIN_RS17925: ECOLIN_RS17925 - 2-ketobutyrate formate-lyase/pyruvate formate-lyase, at 3,613,272 to 3,615,566
_RS17925
ECOLIN_RS17930: ECOLIN_RS17930 - propionate kinase, at 3,615,600 to 3,616,808
_RS17930
Position (kb)
3613
3614
3615
3616 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3612.316 kb on + strand, within ECOLIN_RS17915 at 3612.317 kb on - strand, within ECOLIN_RS17915 at 3612.368 kb on - strand, within ECOLIN_RS17915 at 3612.368 kb on - strand, within ECOLIN_RS17915 at 3612.370 kb on + strand, within ECOLIN_RS17915 at 3612.381 kb on + strand, within ECOLIN_RS17915 at 3612.381 kb on + strand, within ECOLIN_RS17915 at 3612.385 kb on - strand, within ECOLIN_RS17915 at 3612.385 kb on - strand, within ECOLIN_RS17915 at 3612.797 kb on + strand at 3612.882 kb on - strand at 3612.882 kb on - strand at 3612.882 kb on - strand at 3612.882 kb on - strand at 3612.987 kb on + strand, within ECOLIN_RS17920 at 3612.988 kb on - strand, within ECOLIN_RS17920 at 3613.191 kb on - strand, within ECOLIN_RS17920 at 3613.210 kb on - strand, within ECOLIN_RS17920 at 3613.259 kb on - strand at 3613.302 kb on - strand at 3613.302 kb on - strand at 3613.477 kb on + strand at 3613.508 kb on + strand, within ECOLIN_RS17925 at 3613.558 kb on + strand, within ECOLIN_RS17925 at 3613.579 kb on + strand, within ECOLIN_RS17925 at 3613.931 kb on - strand, within ECOLIN_RS17925 at 3613.931 kb on - strand, within ECOLIN_RS17925 at 3614.056 kb on + strand, within ECOLIN_RS17925 at 3614.057 kb on - strand, within ECOLIN_RS17925 at 3614.083 kb on + strand, within ECOLIN_RS17925 at 3614.084 kb on - strand, within ECOLIN_RS17925 at 3614.222 kb on - strand, within ECOLIN_RS17925 at 3614.334 kb on - strand, within ECOLIN_RS17925 at 3614.594 kb on + strand, within ECOLIN_RS17925 at 3614.668 kb on - strand, within ECOLIN_RS17925 at 3614.695 kb on + strand, within ECOLIN_RS17925 at 3614.696 kb on - strand, within ECOLIN_RS17925 at 3614.696 kb on - strand, within ECOLIN_RS17925 at 3614.834 kb on - strand, within ECOLIN_RS17925 at 3614.906 kb on + strand, within ECOLIN_RS17925 at 3615.060 kb on + strand, within ECOLIN_RS17925 at 3615.198 kb on - strand, within ECOLIN_RS17925 at 3615.288 kb on - strand, within ECOLIN_RS17925 at 3615.289 kb on + strand, within ECOLIN_RS17925 at 3615.290 kb on - strand, within ECOLIN_RS17925 at 3615.455 kb on - strand at 3615.583 kb on - strand at 3615.597 kb on - strand at 3615.597 kb on - strand at 3615.810 kb on + strand, within ECOLIN_RS17930 at 3615.845 kb on + strand, within ECOLIN_RS17930 at 3615.972 kb on + strand, within ECOLIN_RS17930 at 3615.995 kb on + strand, within ECOLIN_RS17930 at 3615.999 kb on + strand, within ECOLIN_RS17930 at 3615.999 kb on + strand, within ECOLIN_RS17930 at 3616.016 kb on + strand, within ECOLIN_RS17930 at 3616.303 kb on - strand, within ECOLIN_RS17930 at 3616.341 kb on + strand, within ECOLIN_RS17930 at 3616.455 kb on - strand, within ECOLIN_RS17930 at 3616.509 kb on - strand, within ECOLIN_RS17930
Per-strain Table
Position Strand Gene LocusTag Fraction Bas10 remove 3,612,316 + ECOLIN_RS17915 0.65 +0.3 3,612,317 - ECOLIN_RS17915 0.65 -0.2 3,612,368 - ECOLIN_RS17915 0.68 -1.1 3,612,368 - ECOLIN_RS17915 0.68 -0.5 3,612,370 + ECOLIN_RS17915 0.69 -0.3 3,612,381 + ECOLIN_RS17915 0.69 -0.2 3,612,381 + ECOLIN_RS17915 0.69 -0.5 3,612,385 - ECOLIN_RS17915 0.70 +0.2 3,612,385 - ECOLIN_RS17915 0.70 +0.3 3,612,797 + -0.8 3,612,882 - +0.4 3,612,882 - -0.0 3,612,882 - -0.3 3,612,882 - +0.0 3,612,987 + ECOLIN_RS17920 0.30 +0.0 3,612,988 - ECOLIN_RS17920 0.31 -0.0 3,613,191 - ECOLIN_RS17920 0.83 -0.1 3,613,210 - ECOLIN_RS17920 0.87 -0.0 3,613,259 - +0.2 3,613,302 - -0.0 3,613,302 - +0.1 3,613,477 + -0.6 3,613,508 + ECOLIN_RS17925 0.10 +0.1 3,613,558 + ECOLIN_RS17925 0.12 -0.1 3,613,579 + ECOLIN_RS17925 0.13 -0.2 3,613,931 - ECOLIN_RS17925 0.29 -0.1 3,613,931 - ECOLIN_RS17925 0.29 -0.2 3,614,056 + ECOLIN_RS17925 0.34 +0.2 3,614,057 - ECOLIN_RS17925 0.34 -0.0 3,614,083 + ECOLIN_RS17925 0.35 +0.0 3,614,084 - ECOLIN_RS17925 0.35 -0.1 3,614,222 - ECOLIN_RS17925 0.41 -0.6 3,614,334 - ECOLIN_RS17925 0.46 +0.1 3,614,594 + ECOLIN_RS17925 0.58 -4.1 3,614,668 - ECOLIN_RS17925 0.61 +0.2 3,614,695 + ECOLIN_RS17925 0.62 -0.0 3,614,696 - ECOLIN_RS17925 0.62 -0.3 3,614,696 - ECOLIN_RS17925 0.62 -0.0 3,614,834 - ECOLIN_RS17925 0.68 -0.3 3,614,906 + ECOLIN_RS17925 0.71 -0.0 3,615,060 + ECOLIN_RS17925 0.78 +0.4 3,615,198 - ECOLIN_RS17925 0.84 -0.1 3,615,288 - ECOLIN_RS17925 0.88 +0.5 3,615,289 + ECOLIN_RS17925 0.88 +0.1 3,615,290 - ECOLIN_RS17925 0.88 -1.2 3,615,455 - -0.0 3,615,583 - +1.5 3,615,597 - -0.1 3,615,597 - +0.4 3,615,810 + ECOLIN_RS17930 0.17 -0.2 3,615,845 + ECOLIN_RS17930 0.20 -0.4 3,615,972 + ECOLIN_RS17930 0.31 +0.1 3,615,995 + ECOLIN_RS17930 0.33 +0.4 3,615,999 + ECOLIN_RS17930 0.33 -0.6 3,615,999 + ECOLIN_RS17930 0.33 +0.2 3,616,016 + ECOLIN_RS17930 0.34 -0.5 3,616,303 - ECOLIN_RS17930 0.58 +0.0 3,616,341 + ECOLIN_RS17930 0.61 +0.7 3,616,455 - ECOLIN_RS17930 0.71 -0.4 3,616,509 - ECOLIN_RS17930 0.75 +1.2
Or see this region's nucleotide sequence