Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS01515

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS01500 and ECOLIN_RS32065 are separated by 49 nucleotidesECOLIN_RS32065 and ECOLIN_RS01510 are separated by 67 nucleotidesECOLIN_RS01510 and ECOLIN_RS01515 are separated by 319 nucleotidesECOLIN_RS01515 and ECOLIN_RS01520 are separated by 241 nucleotides ECOLIN_RS01500: ECOLIN_RS01500 - inovirus-type Gp2 protein, at 288,898 to 289,464 _RS01500 ECOLIN_RS32065: ECOLIN_RS32065 - hypothetical protein, at 289,514 to 289,784 _RS32065 ECOLIN_RS01510: ECOLIN_RS01510 - AlpA family transcriptional regulator, at 289,852 to 290,049 _RS01510 ECOLIN_RS01515: ECOLIN_RS01515 - RpiB/LacA/LacB family sugar-phosphate isomerase, at 290,369 to 291,007 _RS01515 ECOLIN_RS01520: ECOLIN_RS01520 - oligogalacturonate lyase family protein, at 291,249 to 292,421 _RS01520 Position (kb) 290 291 292Strain fitness (log2 ratio) -1 0 1 2at 289.388 kb on + strand, within ECOLIN_RS01500at 289.504 kb on - strandat 289.515 kb on + strandat 289.515 kb on + strandat 289.670 kb on + strand, within ECOLIN_RS32065at 289.723 kb on - strand, within ECOLIN_RS32065at 289.860 kb on + strandat 289.919 kb on - strand, within ECOLIN_RS01510at 289.919 kb on - strand, within ECOLIN_RS01510at 290.067 kb on - strandat 290.122 kb on + strandat 290.792 kb on - strand, within ECOLIN_RS01515at 290.901 kb on - strand, within ECOLIN_RS01515

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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289,388 + ECOLIN_RS01500 0.86 -0.1
289,504 - +1.7
289,515 + +0.2
289,515 + +0.1
289,670 + ECOLIN_RS32065 0.58 -0.1
289,723 - ECOLIN_RS32065 0.77 -0.8
289,860 + -1.4
289,919 - ECOLIN_RS01510 0.34 +1.9
289,919 - ECOLIN_RS01510 0.34 +0.4
290,067 - -0.5
290,122 + -0.1
290,792 - ECOLIN_RS01515 0.66 -0.5
290,901 - ECOLIN_RS01515 0.83 -0.2

Or see this region's nucleotide sequence