Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS22525

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS22515 and ECOLIN_RS22525 are separated by 259 nucleotidesECOLIN_RS22525 and ECOLIN_RS22530 are separated by 148 nucleotidesECOLIN_RS22530 and ECOLIN_RS22535 are separated by 105 nucleotides ECOLIN_RS22515: ECOLIN_RS22515 - superoxide dismutase [Mn], at 4,523,607 to 4,524,227 _RS22515 ECOLIN_RS22525: ECOLIN_RS22525 - 2-keto-3-deoxygluconate transporter, at 4,524,487 to 4,525,470 _RS22525 ECOLIN_RS22530: ECOLIN_RS22530 - 6-hydroxyaminopurine reductase, at 4,525,619 to 4,526,293 _RS22530 ECOLIN_RS22535: ECOLIN_RS22535 - envelope stress sensor histidine kinase CpxA, at 4,526,399 to 4,527,772 _RS22535 Position (kb) 4524 4525 4526Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4523.545 kb on - strandat 4523.546 kb on + strandat 4523.606 kb on + strandat 4523.613 kb on + strandat 4523.614 kb on - strandat 4523.709 kb on + strand, within ECOLIN_RS22515at 4523.710 kb on - strand, within ECOLIN_RS22515at 4523.939 kb on + strand, within ECOLIN_RS22515at 4523.940 kb on - strand, within ECOLIN_RS22515at 4524.126 kb on + strand, within ECOLIN_RS22515at 4524.129 kb on + strand, within ECOLIN_RS22515at 4524.130 kb on - strand, within ECOLIN_RS22515at 4524.130 kb on - strand, within ECOLIN_RS22515at 4524.164 kb on + strand, within ECOLIN_RS22515at 4524.495 kb on - strandat 4524.696 kb on - strand, within ECOLIN_RS22525at 4524.703 kb on - strand, within ECOLIN_RS22525at 4524.771 kb on + strand, within ECOLIN_RS22525at 4524.883 kb on + strand, within ECOLIN_RS22525at 4524.954 kb on + strand, within ECOLIN_RS22525at 4525.010 kb on + strand, within ECOLIN_RS22525at 4525.122 kb on + strand, within ECOLIN_RS22525at 4525.333 kb on - strand, within ECOLIN_RS22525at 4525.576 kb on + strandat 4525.576 kb on + strandat 4525.577 kb on - strandat 4525.577 kb on - strandat 4525.577 kb on - strandat 4525.581 kb on - strandat 4525.588 kb on - strandat 4525.638 kb on + strandat 4525.638 kb on + strandat 4525.638 kb on + strandat 4525.638 kb on + strandat 4525.639 kb on - strandat 4525.639 kb on - strandat 4525.639 kb on - strandat 4525.640 kb on + strandat 4525.641 kb on - strandat 4525.641 kb on - strandat 4525.662 kb on - strandat 4525.662 kb on - strandat 4525.811 kb on + strand, within ECOLIN_RS22530at 4525.811 kb on + strand, within ECOLIN_RS22530at 4525.906 kb on + strand, within ECOLIN_RS22530at 4525.907 kb on - strand, within ECOLIN_RS22530at 4525.914 kb on - strand, within ECOLIN_RS22530at 4525.916 kb on - strand, within ECOLIN_RS22530at 4525.924 kb on + strand, within ECOLIN_RS22530at 4525.924 kb on + strand, within ECOLIN_RS22530at 4525.947 kb on + strand, within ECOLIN_RS22530at 4525.948 kb on - strand, within ECOLIN_RS22530at 4525.948 kb on - strand, within ECOLIN_RS22530at 4525.981 kb on + strand, within ECOLIN_RS22530at 4526.014 kb on - strand, within ECOLIN_RS22530at 4526.055 kb on - strand, within ECOLIN_RS22530at 4526.055 kb on - strand, within ECOLIN_RS22530at 4526.055 kb on - strand, within ECOLIN_RS22530at 4526.055 kb on - strand, within ECOLIN_RS22530at 4526.056 kb on + strand, within ECOLIN_RS22530at 4526.061 kb on + strand, within ECOLIN_RS22530at 4526.062 kb on - strand, within ECOLIN_RS22530at 4526.181 kb on - strand, within ECOLIN_RS22530at 4526.410 kb on + strandat 4526.410 kb on + strandat 4526.412 kb on + strandat 4526.412 kb on + strandat 4526.413 kb on - strandat 4526.413 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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4,523,545 - +0.1
4,523,546 + +0.4
4,523,606 + +0.7
4,523,613 + -0.1
4,523,614 - +0.0
4,523,709 + ECOLIN_RS22515 0.16 +0.1
4,523,710 - ECOLIN_RS22515 0.17 -0.4
4,523,939 + ECOLIN_RS22515 0.53 +0.2
4,523,940 - ECOLIN_RS22515 0.54 +0.1
4,524,126 + ECOLIN_RS22515 0.84 -0.0
4,524,129 + ECOLIN_RS22515 0.84 +0.2
4,524,130 - ECOLIN_RS22515 0.84 -0.5
4,524,130 - ECOLIN_RS22515 0.84 +0.1
4,524,164 + ECOLIN_RS22515 0.90 -0.1
4,524,495 - +0.0
4,524,696 - ECOLIN_RS22525 0.21 +0.2
4,524,703 - ECOLIN_RS22525 0.22 -0.6
4,524,771 + ECOLIN_RS22525 0.29 +0.2
4,524,883 + ECOLIN_RS22525 0.40 +0.2
4,524,954 + ECOLIN_RS22525 0.47 +0.3
4,525,010 + ECOLIN_RS22525 0.53 +0.0
4,525,122 + ECOLIN_RS22525 0.65 -0.1
4,525,333 - ECOLIN_RS22525 0.86 +0.1
4,525,576 + +0.0
4,525,576 + -0.2
4,525,577 - -0.4
4,525,577 - +0.2
4,525,577 - -0.2
4,525,581 - +0.1
4,525,588 - +0.0
4,525,638 + -0.1
4,525,638 + +0.2
4,525,638 + -0.1
4,525,638 + -0.5
4,525,639 - +1.7
4,525,639 - +0.1
4,525,639 - -1.0
4,525,640 + +0.3
4,525,641 - +0.5
4,525,641 - +0.2
4,525,662 - +0.4
4,525,662 - +0.1
4,525,811 + ECOLIN_RS22530 0.28 -0.2
4,525,811 + ECOLIN_RS22530 0.28 +0.6
4,525,906 + ECOLIN_RS22530 0.43 +0.1
4,525,907 - ECOLIN_RS22530 0.43 +0.5
4,525,914 - ECOLIN_RS22530 0.44 +0.2
4,525,916 - ECOLIN_RS22530 0.44 -0.6
4,525,924 + ECOLIN_RS22530 0.45 -0.1
4,525,924 + ECOLIN_RS22530 0.45 -0.5
4,525,947 + ECOLIN_RS22530 0.49 -0.6
4,525,948 - ECOLIN_RS22530 0.49 +0.7
4,525,948 - ECOLIN_RS22530 0.49 -0.2
4,525,981 + ECOLIN_RS22530 0.54 -0.1
4,526,014 - ECOLIN_RS22530 0.59 +0.0
4,526,055 - ECOLIN_RS22530 0.65 -0.0
4,526,055 - ECOLIN_RS22530 0.65 +0.0
4,526,055 - ECOLIN_RS22530 0.65 -0.2
4,526,055 - ECOLIN_RS22530 0.65 -0.4
4,526,056 + ECOLIN_RS22530 0.65 -0.2
4,526,061 + ECOLIN_RS22530 0.65 +0.4
4,526,062 - ECOLIN_RS22530 0.66 -0.3
4,526,181 - ECOLIN_RS22530 0.83 +0.2
4,526,410 + -5.1
4,526,410 + +0.0
4,526,412 + -0.1
4,526,412 + +0.2
4,526,413 - +0.1
4,526,413 - +0.7

Or see this region's nucleotide sequence