Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS17195

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS17185 and ECOLIN_RS17190 overlap by 1 nucleotidesECOLIN_RS17190 and ECOLIN_RS17195 overlap by 1 nucleotidesECOLIN_RS17195 and ECOLIN_RS17200 are separated by 45 nucleotidesECOLIN_RS17200 and ECOLIN_RS17205 are separated by 31 nucleotides ECOLIN_RS17185: ECOLIN_RS17185 - YtfJ family protein, at 3,465,263 to 3,465,823 _RS17185 ECOLIN_RS17190: ECOLIN_RS17190 - hypothetical protein, at 3,465,823 to 3,466,659 _RS17190 ECOLIN_RS17195: ECOLIN_RS17195 - lipopolysaccharide biosynthesis protein, at 3,466,659 to 3,468,008 _RS17195 ECOLIN_RS17200: ECOLIN_RS17200 - hypothetical protein, at 3,468,054 to 3,468,812 _RS17200 ECOLIN_RS17205: ECOLIN_RS17205 - dTMP kinase, at 3,468,844 to 3,469,557 _RS17205 Position (kb) 3466 3467 3468 3469Strain fitness (log2 ratio) -2 -1 0 1at 3465.855 kb on + strandat 3465.909 kb on - strand, within ECOLIN_RS17190at 3465.928 kb on - strand, within ECOLIN_RS17190at 3465.954 kb on + strand, within ECOLIN_RS17190at 3466.011 kb on + strand, within ECOLIN_RS17190at 3466.044 kb on - strand, within ECOLIN_RS17190at 3466.100 kb on - strand, within ECOLIN_RS17190at 3466.200 kb on - strand, within ECOLIN_RS17190at 3466.233 kb on - strand, within ECOLIN_RS17190at 3466.354 kb on + strand, within ECOLIN_RS17190at 3466.404 kb on - strand, within ECOLIN_RS17190at 3466.524 kb on + strand, within ECOLIN_RS17190at 3466.683 kb on - strandat 3467.071 kb on - strand, within ECOLIN_RS17195at 3467.137 kb on - strand, within ECOLIN_RS17195at 3467.308 kb on + strand, within ECOLIN_RS17195at 3467.389 kb on + strand, within ECOLIN_RS17195at 3467.458 kb on + strand, within ECOLIN_RS17195at 3467.511 kb on + strand, within ECOLIN_RS17195at 3467.511 kb on + strand, within ECOLIN_RS17195at 3467.631 kb on + strand, within ECOLIN_RS17195at 3467.841 kb on + strand, within ECOLIN_RS17195at 3467.841 kb on + strand, within ECOLIN_RS17195at 3468.032 kb on - strandat 3468.055 kb on + strandat 3468.056 kb on - strandat 3468.056 kb on - strandat 3468.188 kb on + strand, within ECOLIN_RS17200at 3468.189 kb on - strand, within ECOLIN_RS17200at 3468.189 kb on - strand, within ECOLIN_RS17200at 3468.189 kb on - strand, within ECOLIN_RS17200at 3468.276 kb on + strand, within ECOLIN_RS17200at 3468.499 kb on + strand, within ECOLIN_RS17200at 3468.500 kb on - strand, within ECOLIN_RS17200at 3468.500 kb on - strand, within ECOLIN_RS17200at 3468.503 kb on + strand, within ECOLIN_RS17200at 3468.758 kb on - strandat 3468.758 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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3,465,855 + +0.2
3,465,909 - ECOLIN_RS17190 0.10 -0.1
3,465,928 - ECOLIN_RS17190 0.13 +1.1
3,465,954 + ECOLIN_RS17190 0.16 +0.1
3,466,011 + ECOLIN_RS17190 0.22 +1.4
3,466,044 - ECOLIN_RS17190 0.26 +0.0
3,466,100 - ECOLIN_RS17190 0.33 +0.8
3,466,200 - ECOLIN_RS17190 0.45 +1.5
3,466,233 - ECOLIN_RS17190 0.49 +0.4
3,466,354 + ECOLIN_RS17190 0.63 +0.2
3,466,404 - ECOLIN_RS17190 0.69 +0.2
3,466,524 + ECOLIN_RS17190 0.84 -0.0
3,466,683 - +0.5
3,467,071 - ECOLIN_RS17195 0.31 -1.8
3,467,137 - ECOLIN_RS17195 0.35 +1.7
3,467,308 + ECOLIN_RS17195 0.48 -2.1
3,467,389 + ECOLIN_RS17195 0.54 -0.5
3,467,458 + ECOLIN_RS17195 0.59 +0.2
3,467,511 + ECOLIN_RS17195 0.63 -0.0
3,467,511 + ECOLIN_RS17195 0.63 -0.1
3,467,631 + ECOLIN_RS17195 0.72 +0.4
3,467,841 + ECOLIN_RS17195 0.88 -0.0
3,467,841 + ECOLIN_RS17195 0.88 +1.1
3,468,032 - +0.0
3,468,055 + +0.1
3,468,056 - +0.1
3,468,056 - -0.1
3,468,188 + ECOLIN_RS17200 0.18 +0.1
3,468,189 - ECOLIN_RS17200 0.18 -0.4
3,468,189 - ECOLIN_RS17200 0.18 -0.0
3,468,189 - ECOLIN_RS17200 0.18 -0.0
3,468,276 + ECOLIN_RS17200 0.29 +0.5
3,468,499 + ECOLIN_RS17200 0.59 +0.0
3,468,500 - ECOLIN_RS17200 0.59 +0.6
3,468,500 - ECOLIN_RS17200 0.59 -0.5
3,468,503 + ECOLIN_RS17200 0.59 -1.2
3,468,758 - -0.1
3,468,758 - +0.3

Or see this region's nucleotide sequence