Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS14250

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS14245 and ECOLIN_RS14250 are separated by 68 nucleotidesECOLIN_RS14250 and ECOLIN_RS14255 are separated by 120 nucleotides ECOLIN_RS14245: ECOLIN_RS14245 - exodeoxyribonuclease VII large subunit, at 2,863,262 to 2,864,638 _RS14245 ECOLIN_RS14250: ECOLIN_RS14250 - DUF823 domain-containing adhesin, at 2,864,707 to 2,872,758 _RS14250 ECOLIN_RS14255: ECOLIN_RS14255 - SinI family autotransporter-associated protein, at 2,872,879 to 2,873,856 _RS14255 Position (kb) 2864 2865 2866 2867 2868 2869 2870 2871 2872 2873Strain fitness (log2 ratio) -1 0 1 2at 2863.719 kb on + strand, within ECOLIN_RS14245at 2863.726 kb on + strand, within ECOLIN_RS14245at 2863.726 kb on + strand, within ECOLIN_RS14245at 2863.909 kb on + strand, within ECOLIN_RS14245at 2864.213 kb on - strand, within ECOLIN_RS14245at 2865.644 kb on + strand, within ECOLIN_RS14250at 2865.645 kb on - strand, within ECOLIN_RS14250at 2865.798 kb on + strand, within ECOLIN_RS14250at 2866.525 kb on - strand, within ECOLIN_RS14250at 2866.525 kb on - strand, within ECOLIN_RS14250at 2866.570 kb on - strand, within ECOLIN_RS14250at 2866.572 kb on - strand, within ECOLIN_RS14250at 2866.572 kb on - strand, within ECOLIN_RS14250at 2866.572 kb on - strand, within ECOLIN_RS14250at 2866.574 kb on - strand, within ECOLIN_RS14250at 2866.956 kb on + strand, within ECOLIN_RS14250at 2867.451 kb on - strand, within ECOLIN_RS14250at 2868.159 kb on + strand, within ECOLIN_RS14250at 2868.200 kb on + strand, within ECOLIN_RS14250at 2868.293 kb on + strand, within ECOLIN_RS14250at 2868.433 kb on - strand, within ECOLIN_RS14250at 2869.500 kb on - strand, within ECOLIN_RS14250at 2869.612 kb on - strand, within ECOLIN_RS14250at 2869.943 kb on - strand, within ECOLIN_RS14250at 2870.246 kb on + strand, within ECOLIN_RS14250at 2870.454 kb on + strand, within ECOLIN_RS14250at 2871.276 kb on - strand, within ECOLIN_RS14250at 2872.177 kb on - strandat 2872.267 kb on + strandat 2872.910 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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2,863,719 + ECOLIN_RS14245 0.33 +0.0
2,863,726 + ECOLIN_RS14245 0.34 +0.5
2,863,726 + ECOLIN_RS14245 0.34 +1.8
2,863,909 + ECOLIN_RS14245 0.47 +0.2
2,864,213 - ECOLIN_RS14245 0.69 +0.0
2,865,644 + ECOLIN_RS14250 0.12 -0.1
2,865,645 - ECOLIN_RS14250 0.12 -1.6
2,865,798 + ECOLIN_RS14250 0.14 +0.2
2,866,525 - ECOLIN_RS14250 0.23 +0.1
2,866,525 - ECOLIN_RS14250 0.23 +0.4
2,866,570 - ECOLIN_RS14250 0.23 +0.7
2,866,572 - ECOLIN_RS14250 0.23 +1.1
2,866,572 - ECOLIN_RS14250 0.23 -0.5
2,866,572 - ECOLIN_RS14250 0.23 +0.3
2,866,574 - ECOLIN_RS14250 0.23 +0.4
2,866,956 + ECOLIN_RS14250 0.28 -0.4
2,867,451 - ECOLIN_RS14250 0.34 -0.0
2,868,159 + ECOLIN_RS14250 0.43 +0.9
2,868,200 + ECOLIN_RS14250 0.43 -0.6
2,868,293 + ECOLIN_RS14250 0.45 -0.3
2,868,433 - ECOLIN_RS14250 0.46 +0.3
2,869,500 - ECOLIN_RS14250 0.60 +0.3
2,869,612 - ECOLIN_RS14250 0.61 -0.2
2,869,943 - ECOLIN_RS14250 0.65 -0.8
2,870,246 + ECOLIN_RS14250 0.69 -0.1
2,870,454 + ECOLIN_RS14250 0.71 +0.7
2,871,276 - ECOLIN_RS14250 0.82 +1.4
2,872,177 - +0.7
2,872,267 + +1.2
2,872,910 + -0.2

Or see this region's nucleotide sequence