Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS08585

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS08580 and ECOLIN_RS08585 are separated by 37 nucleotidesECOLIN_RS08585 and ECOLIN_RS31420 are separated by 199 nucleotidesECOLIN_RS31420 and ECOLIN_RS08590 overlap by 35 nucleotides ECOLIN_RS08580: ECOLIN_RS08580 - TonB-dependent receptor plug domain-containing protein, at 1,688,976 to 1,691,348 _RS08580 ECOLIN_RS08585: ECOLIN_RS08585 - ABC transporter ATP-binding protein/permease, at 1,691,386 to 1,693,071 _RS08585 ECOLIN_RS31420: ECOLIN_RS31420 - hypothetical protein, at 1,693,271 to 1,693,396 _RS31420 ECOLIN_RS08590: ECOLIN_RS08590 - anaerobic sulfatase maturase, at 1,693,362 to 1,694,519 _RS08590 Position (kb) 1691 1692 1693 1694Strain fitness (log2 ratio) -1 0 1at 1690.446 kb on - strand, within ECOLIN_RS08580at 1690.446 kb on - strand, within ECOLIN_RS08580at 1691.016 kb on - strand, within ECOLIN_RS08580at 1691.566 kb on + strand, within ECOLIN_RS08585

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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1,690,446 - ECOLIN_RS08580 0.62 -0.0
1,690,446 - ECOLIN_RS08580 0.62 +0.4
1,691,016 - ECOLIN_RS08580 0.86 +0.2
1,691,566 + ECOLIN_RS08585 0.11 -0.6

Or see this region's nucleotide sequence