Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS02550

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS02540 and ECOLIN_RS02545 overlap by 1 nucleotidesECOLIN_RS02545 and ECOLIN_RS02550 are separated by 205 nucleotidesECOLIN_RS02550 and ECOLIN_RS02555 are separated by 53 nucleotidesECOLIN_RS02555 and ECOLIN_RS02560 overlap by 38 nucleotides ECOLIN_RS02540: ECOLIN_RS02540 - (2E,6E)-farnesyl diphosphate synthase, at 519,508 to 520,407 _RS02540 ECOLIN_RS02545: ECOLIN_RS02545 - exodeoxyribonuclease VII small subunit, at 520,407 to 520,649 _RS02545 ECOLIN_RS02550: ECOLIN_RS02550 - tRNA uracil 4-sulfurtransferase ThiI, at 520,855 to 522,303 _RS02550 ECOLIN_RS02555: ECOLIN_RS02555 - protein deglycase YajL, at 522,357 to 522,947 _RS02555 ECOLIN_RS02560: ECOLIN_RS02560 - 2-dehydropantoate 2-reductase, at 522,910 to 523,821 _RS02560 Position (kb) 520 521 522 523Strain fitness (log2 ratio) -1 0 1at 520.561 kb on - strand, within ECOLIN_RS02545at 520.729 kb on - strandat 520.827 kb on + strandat 520.827 kb on + strandat 520.963 kb on + strandat 521.259 kb on + strand, within ECOLIN_RS02550at 521.259 kb on + strand, within ECOLIN_RS02550at 521.358 kb on + strand, within ECOLIN_RS02550at 521.581 kb on - strand, within ECOLIN_RS02550at 521.652 kb on + strand, within ECOLIN_RS02550at 521.652 kb on + strand, within ECOLIN_RS02550at 522.276 kb on + strandat 522.292 kb on + strandat 523.159 kb on - strand, within ECOLIN_RS02560at 523.159 kb on - strand, within ECOLIN_RS02560at 523.201 kb on + strand, within ECOLIN_RS02560

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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520,561 - ECOLIN_RS02545 0.63 -1.0
520,729 - -0.4
520,827 + -0.0
520,827 + +0.0
520,963 + +1.5
521,259 + ECOLIN_RS02550 0.28 -1.6
521,259 + ECOLIN_RS02550 0.28 +0.4
521,358 + ECOLIN_RS02550 0.35 -0.3
521,581 - ECOLIN_RS02550 0.50 -0.3
521,652 + ECOLIN_RS02550 0.55 -0.2
521,652 + ECOLIN_RS02550 0.55 -0.8
522,276 + +0.6
522,292 + -0.2
523,159 - ECOLIN_RS02560 0.27 -0.1
523,159 - ECOLIN_RS02560 0.27 -0.6
523,201 + ECOLIN_RS02560 0.32 +0.1

Or see this region's nucleotide sequence