Experiment: Formate/Acetate-Sulfate (60/30-30 mM) minimal without Molybdate/Tungstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phsA and DVU0174 are separated by 82 nucleotides DVU0174 and DVU0175 are separated by 476 nucleotides
DVU0173: phsA - thiosulfate reductase (Shelley Haveman), at 216,495 to 218,591
phsA
DVU0174: DVU0174 - hypothetical protein (TIGR), at 218,674 to 218,781
DVU0174
DVU0175: DVU0175 - tungsten formylmethanofuran dehydrogenase family protein/molybdopterin binding protein (TIGR), at 219,258 to 220,889
DVU0175
Position (kb) 218
219 Strain fitness (log2 ratio) -2
-1
0
1
2 at 217.707 kb on + strand, within phsA at 217.739 kb on - strand, within phsA at 217.798 kb on + strand, within phsA at 217.823 kb on - strand, within phsA at 217.842 kb on - strand, within phsA at 217.844 kb on + strand, within phsA at 217.897 kb on - strand, within phsA at 217.923 kb on + strand, within phsA at 217.947 kb on - strand, within phsA at 218.044 kb on + strand, within phsA at 218.044 kb on + strand, within phsA at 218.052 kb on - strand, within phsA at 218.057 kb on - strand, within phsA at 218.062 kb on - strand, within phsA at 218.093 kb on - strand, within phsA at 218.118 kb on + strand, within phsA at 218.126 kb on - strand, within phsA at 218.216 kb on + strand, within phsA at 218.224 kb on - strand, within phsA at 218.243 kb on - strand, within phsA at 218.262 kb on + strand, within phsA at 218.389 kb on + strand at 218.468 kb on - strand at 218.477 kb on - strand at 218.485 kb on + strand at 218.493 kb on - strand at 218.524 kb on - strand at 218.531 kb on + strand at 218.539 kb on - strand at 218.539 kb on - strand at 218.607 kb on + strand at 218.609 kb on + strand at 218.617 kb on - strand at 218.639 kb on + strand at 218.648 kb on + strand at 218.681 kb on - strand at 218.683 kb on + strand at 218.688 kb on + strand, within DVU0174 at 218.693 kb on + strand, within DVU0174 at 218.696 kb on - strand, within DVU0174 at 218.698 kb on + strand, within DVU0174 at 218.698 kb on + strand, within DVU0174 at 218.713 kb on - strand, within DVU0174 at 218.933 kb on + strand at 218.948 kb on + strand at 218.953 kb on + strand at 218.953 kb on + strand at 218.961 kb on - strand at 218.997 kb on + strand at 219.000 kb on - strand at 219.035 kb on - strand at 219.037 kb on - strand at 219.038 kb on + strand at 219.130 kb on + strand at 219.264 kb on - strand at 219.264 kb on - strand at 219.264 kb on - strand at 219.264 kb on - strand at 219.279 kb on + strand at 219.330 kb on - strand at 219.338 kb on + strand at 219.386 kb on + strand at 219.387 kb on + strand at 219.395 kb on - strand at 219.430 kb on + strand, within DVU0175 at 219.430 kb on + strand, within DVU0175 at 219.438 kb on - strand, within DVU0175 at 219.472 kb on + strand, within DVU0175 at 219.472 kb on + strand, within DVU0175 at 219.512 kb on + strand, within DVU0175 at 219.582 kb on - strand, within DVU0175 at 219.651 kb on - strand, within DVU0175 at 219.688 kb on - strand, within DVU0175 at 219.727 kb on + strand, within DVU0175
Per-strain Table
Position Strand Gene LocusTag Fraction Formate/Acetate-Sulfate (60/30-30 mM) minimal without Molybdate/Tungstate remove 217,707 + phsA DVU0173 0.58 -0.3 217,739 - phsA DVU0173 0.59 +0.3 217,798 + phsA DVU0173 0.62 +0.1 217,823 - phsA DVU0173 0.63 +0.1 217,842 - phsA DVU0173 0.64 -0.4 217,844 + phsA DVU0173 0.64 +1.5 217,897 - phsA DVU0173 0.67 -0.6 217,923 + phsA DVU0173 0.68 +0.2 217,947 - phsA DVU0173 0.69 +0.4 218,044 + phsA DVU0173 0.74 +0.0 218,044 + phsA DVU0173 0.74 -0.2 218,052 - phsA DVU0173 0.74 -0.3 218,057 - phsA DVU0173 0.74 -0.7 218,062 - phsA DVU0173 0.75 -1.5 218,093 - phsA DVU0173 0.76 +0.2 218,118 + phsA DVU0173 0.77 -0.3 218,126 - phsA DVU0173 0.78 +1.8 218,216 + phsA DVU0173 0.82 -0.3 218,224 - phsA DVU0173 0.82 -0.4 218,243 - phsA DVU0173 0.83 -1.0 218,262 + phsA DVU0173 0.84 -0.1 218,389 + -0.2 218,468 - -0.1 218,477 - -0.3 218,485 + -0.7 218,493 - -0.3 218,524 - +0.1 218,531 + -0.3 218,539 - +0.3 218,539 - -0.2 218,607 + +0.2 218,609 + -0.2 218,617 - -0.6 218,639 + +0.5 218,648 + -0.2 218,681 - -0.2 218,683 + -0.8 218,688 + DVU0174 0.13 -0.8 218,693 + DVU0174 0.18 -0.8 218,696 - DVU0174 0.20 +0.3 218,698 + DVU0174 0.22 -0.5 218,698 + DVU0174 0.22 -1.1 218,713 - DVU0174 0.36 -1.5 218,933 + -0.3 218,948 + +0.3 218,953 + -0.0 218,953 + -0.3 218,961 - -0.4 218,997 + -0.1 219,000 - -0.1 219,035 - +0.4 219,037 - -0.2 219,038 + +0.1 219,130 + -0.1 219,264 - -0.3 219,264 - +0.2 219,264 - -1.0 219,264 - +1.0 219,279 + -0.7 219,330 - -0.5 219,338 + -0.3 219,386 + -0.0 219,387 + -0.1 219,395 - -0.2 219,430 + DVU0175 0.11 -0.2 219,430 + DVU0175 0.11 -0.1 219,438 - DVU0175 0.11 -0.2 219,472 + DVU0175 0.13 -1.3 219,472 + DVU0175 0.13 -0.6 219,512 + DVU0175 0.16 -0.5 219,582 - DVU0175 0.20 +0.1 219,651 - DVU0175 0.24 -0.5 219,688 - DVU0175 0.26 -1.2 219,727 + DVU0175 0.29 -2.0
Or see this region's nucleotide sequence