Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS14975

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS14970 and ECOLIN_RS14975 are separated by 257 nucleotidesECOLIN_RS14975 and ECOLIN_RS14980 overlap by 4 nucleotides ECOLIN_RS14970: ECOLIN_RS14970 - multidrug efflux MFS transporter permease subunit EmrB, at 3,020,837 to 3,022,375 _RS14970 ECOLIN_RS14975: ECOLIN_RS14975 - RNA ligase family protein, at 3,022,633 to 3,023,330 _RS14975 ECOLIN_RS14980: ECOLIN_RS14980 - ATP-binding protein, at 3,023,327 to 3,024,439 _RS14980 Position (kb) 3022 3023 3024Strain fitness (log2 ratio) -1 0 1 2 3at 3021.862 kb on + strand, within ECOLIN_RS14970at 3022.175 kb on + strand, within ECOLIN_RS14970at 3023.232 kb on + strand, within ECOLIN_RS14975at 3023.232 kb on + strand, within ECOLIN_RS14975at 3023.479 kb on + strand, within ECOLIN_RS14980at 3023.590 kb on + strand, within ECOLIN_RS14980at 3024.142 kb on + strand, within ECOLIN_RS14980

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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3,021,862 + ECOLIN_RS14970 0.67 +2.8
3,022,175 + ECOLIN_RS14970 0.87 +0.2
3,023,232 + ECOLIN_RS14975 0.86 -1.7
3,023,232 + ECOLIN_RS14975 0.86 +1.8
3,023,479 + ECOLIN_RS14980 0.14 -0.8
3,023,590 + ECOLIN_RS14980 0.24 +0.5
3,024,142 + ECOLIN_RS14980 0.73 +0.2

Or see this region's nucleotide sequence