Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS10735

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS10725 and ECOLIN_RS10730 are separated by 52 nucleotidesECOLIN_RS10730 and ECOLIN_RS10735 overlap by 1 nucleotidesECOLIN_RS10735 and ECOLIN_RS10740 overlap by 8 nucleotides ECOLIN_RS10725: ECOLIN_RS10725 - arginine--tRNA ligase, at 2,087,778 to 2,089,511 _RS10725 ECOLIN_RS10730: ECOLIN_RS10730 - flagellar protein FlhE, at 2,089,564 to 2,089,956 _RS10730 ECOLIN_RS10735: ECOLIN_RS10735 - flagellar biosynthesis protein FlhA, at 2,089,956 to 2,092,034 _RS10735 ECOLIN_RS10740: ECOLIN_RS10740 - flagellar biosynthesis protein FlhB, at 2,092,027 to 2,093,175 _RS10740 Position (kb) 2089 2090 2091 2092 2093Strain fitness (log2 ratio) -2 -1 0 1at 2091.558 kb on - strand, within ECOLIN_RS10735at 2092.969 kb on - strand, within ECOLIN_RS10740at 2092.969 kb on - strand, within ECOLIN_RS10740

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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2,091,558 - ECOLIN_RS10735 0.77 -1.8
2,092,969 - ECOLIN_RS10740 0.82 +0.9
2,092,969 - ECOLIN_RS10740 0.82 +0.6

Or see this region's nucleotide sequence