Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS08580

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS08575 and ECOLIN_RS08580 are separated by 56 nucleotidesECOLIN_RS08580 and ECOLIN_RS08585 are separated by 37 nucleotides ECOLIN_RS08575: ECOLIN_RS08575 - M16 family metallopeptidase, at 1,686,136 to 1,688,919 _RS08575 ECOLIN_RS08580: ECOLIN_RS08580 - TonB-dependent receptor plug domain-containing protein, at 1,688,976 to 1,691,348 _RS08580 ECOLIN_RS08585: ECOLIN_RS08585 - ABC transporter ATP-binding protein/permease, at 1,691,386 to 1,693,071 _RS08585 Position (kb) 1688 1689 1690 1691 1692Strain fitness (log2 ratio) -2 -1 0 1at 1688.190 kb on + strand, within ECOLIN_RS08575at 1688.680 kb on - strandat 1690.446 kb on - strand, within ECOLIN_RS08580at 1690.446 kb on - strand, within ECOLIN_RS08580at 1691.016 kb on - strand, within ECOLIN_RS08580at 1691.566 kb on + strand, within ECOLIN_RS08585

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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1,688,190 + ECOLIN_RS08575 0.74 -1.9
1,688,680 - -0.4
1,690,446 - ECOLIN_RS08580 0.62 -1.9
1,690,446 - ECOLIN_RS08580 0.62 -0.5
1,691,016 - ECOLIN_RS08580 0.86 -0.3
1,691,566 + ECOLIN_RS08585 0.11 -0.3

Or see this region's nucleotide sequence