Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS05290

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS05280 and ECOLIN_RS05285 are separated by 287 nucleotidesECOLIN_RS05285 and ECOLIN_RS05290 are separated by 39 nucleotidesECOLIN_RS05290 and ECOLIN_RS05295 are separated by 54 nucleotidesECOLIN_RS05295 and ECOLIN_RS05300 are separated by 158 nucleotides ECOLIN_RS05280: ECOLIN_RS05280 - pyrimidine utilization protein A, at 1,079,035 to 1,080,126 _RS05280 ECOLIN_RS05285: ECOLIN_RS05285 - HTH-type transcriptional regulator RutR, at 1,080,414 to 1,081,052 _RS05285 ECOLIN_RS05290: ECOLIN_RS05290 - trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase, at 1,081,092 to 1,085,054 _RS05290 ECOLIN_RS05295: ECOLIN_RS05295 - hypothetical protein, at 1,085,109 to 1,085,318 _RS05295 ECOLIN_RS05300: ECOLIN_RS05300 - sodium/proline symporter PutP, at 1,085,477 to 1,086,985 _RS05300 Position (kb) 1081 1082 1083 1084 1085 1086Strain fitness (log2 ratio) -1 0 1at 1081.838 kb on - strand, within ECOLIN_RS05290at 1082.127 kb on - strand, within ECOLIN_RS05290at 1082.127 kb on - strand, within ECOLIN_RS05290at 1082.142 kb on - strand, within ECOLIN_RS05290at 1082.798 kb on - strand, within ECOLIN_RS05290at 1083.063 kb on - strand, within ECOLIN_RS05290at 1084.200 kb on - strand, within ECOLIN_RS05290at 1084.897 kb on - strandat 1085.030 kb on + strandat 1085.474 kb on + strandat 1085.482 kb on + strandat 1085.516 kb on + strandat 1085.579 kb on + strandat 1085.579 kb on + strandat 1085.894 kb on + strand, within ECOLIN_RS05300

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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1,081,838 - ECOLIN_RS05290 0.19 +0.4
1,082,127 - ECOLIN_RS05290 0.26 -0.0
1,082,127 - ECOLIN_RS05290 0.26 -0.4
1,082,142 - ECOLIN_RS05290 0.26 +0.1
1,082,798 - ECOLIN_RS05290 0.43 -0.7
1,083,063 - ECOLIN_RS05290 0.50 +0.0
1,084,200 - ECOLIN_RS05290 0.78 -0.7
1,084,897 - +0.3
1,085,030 + -0.2
1,085,474 + +0.6
1,085,482 + -0.1
1,085,516 + +0.1
1,085,579 + -0.7
1,085,579 + +1.3
1,085,894 + ECOLIN_RS05300 0.28 -0.7

Or see this region's nucleotide sequence