Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS02385

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS02375 and ECOLIN_RS02380 are separated by 147 nucleotidesECOLIN_RS02380 and ECOLIN_RS31260 overlap by 4 nucleotidesECOLIN_RS31260 and ECOLIN_RS02385 are separated by 80 nucleotidesECOLIN_RS02385 and ECOLIN_RS02390 are separated by 124 nucleotides ECOLIN_RS02375: ECOLIN_RS02375 - pyrimidine/purine nucleoside phosphorylase, at 482,970 to 483,254 _RS02375 ECOLIN_RS02380: ECOLIN_RS02380 - DUF2773 domain-containing protein, at 483,402 to 483,743 _RS02380 ECOLIN_RS31260: ECOLIN_RS31260 - hypothetical protein, at 483,740 to 483,820 _RS31260 ECOLIN_RS02385: ECOLIN_RS02385 - recombination-associated protein RdgC, at 483,901 to 484,812 _RS02385 ECOLIN_RS02390: ECOLIN_RS02390 - fructokinase, at 484,937 to 485,845 _RS02390 Position (kb) 483 484 485Strain fitness (log2 ratio) -1 0 1at 483.072 kb on + strand, within ECOLIN_RS02375at 483.133 kb on + strand, within ECOLIN_RS02375at 483.576 kb on - strand, within ECOLIN_RS02380at 484.322 kb on - strand, within ECOLIN_RS02385at 484.366 kb on - strand, within ECOLIN_RS02385at 484.402 kb on - strand, within ECOLIN_RS02385at 484.785 kb on - strandat 485.211 kb on + strand, within ECOLIN_RS02390at 485.211 kb on + strand, within ECOLIN_RS02390at 485.706 kb on + strand, within ECOLIN_RS02390at 485.717 kb on + strand, within ECOLIN_RS02390

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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483,072 + ECOLIN_RS02375 0.36 -0.4
483,133 + ECOLIN_RS02375 0.57 -0.2
483,576 - ECOLIN_RS02380 0.51 +0.4
484,322 - ECOLIN_RS02385 0.46 +0.2
484,366 - ECOLIN_RS02385 0.51 +1.1
484,402 - ECOLIN_RS02385 0.55 +0.8
484,785 - +0.2
485,211 + ECOLIN_RS02390 0.30 +0.1
485,211 + ECOLIN_RS02390 0.30 +0.1
485,706 + ECOLIN_RS02390 0.85 +0.4
485,717 + ECOLIN_RS02390 0.86 -0.2

Or see this region's nucleotide sequence