Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS23465

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS23460 and ECOLIN_RS23465 overlap by 8 nucleotidesECOLIN_RS23465 and ECOLIN_RS23470 are separated by 276 nucleotides ECOLIN_RS23460: ECOLIN_RS23460 - sigma-54 dependent transcriptional regulator, at 4,725,700 to 4,727,058 _RS23460 ECOLIN_RS23465: ECOLIN_RS23465 - ATP-binding protein, at 4,727,051 to 4,728,871 _RS23465 ECOLIN_RS23470: ECOLIN_RS23470 - acid phosphatase AphA, at 4,729,148 to 4,729,861 _RS23470 Position (kb) 4727 4728 4729Strain fitness (log2 ratio) -1 0 1 2 3 4at 4726.087 kb on - strand, within ECOLIN_RS23460at 4726.186 kb on + strand, within ECOLIN_RS23460at 4726.542 kb on + strand, within ECOLIN_RS23460at 4726.543 kb on - strand, within ECOLIN_RS23460at 4727.481 kb on + strand, within ECOLIN_RS23465at 4727.543 kb on - strand, within ECOLIN_RS23465at 4727.635 kb on + strand, within ECOLIN_RS23465at 4727.840 kb on - strand, within ECOLIN_RS23465at 4728.485 kb on + strand, within ECOLIN_RS23465at 4728.963 kb on + strandat 4729.031 kb on - strandat 4729.049 kb on + strandat 4729.252 kb on + strand, within ECOLIN_RS23470at 4729.253 kb on - strand, within ECOLIN_RS23470at 4729.316 kb on - strand, within ECOLIN_RS23470at 4729.672 kb on + strand, within ECOLIN_RS23470at 4729.696 kb on + strand, within ECOLIN_RS23470at 4729.800 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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4,726,087 - ECOLIN_RS23460 0.28 +0.6
4,726,186 + ECOLIN_RS23460 0.36 -0.0
4,726,542 + ECOLIN_RS23460 0.62 -0.7
4,726,543 - ECOLIN_RS23460 0.62 +1.4
4,727,481 + ECOLIN_RS23465 0.24 -0.2
4,727,543 - ECOLIN_RS23465 0.27 +0.1
4,727,635 + ECOLIN_RS23465 0.32 -0.7
4,727,840 - ECOLIN_RS23465 0.43 +0.2
4,728,485 + ECOLIN_RS23465 0.79 -1.0
4,728,963 + -0.2
4,729,031 - -0.4
4,729,049 + -0.7
4,729,252 + ECOLIN_RS23470 0.15 +0.3
4,729,253 - ECOLIN_RS23470 0.15 +0.5
4,729,316 - ECOLIN_RS23470 0.24 -0.1
4,729,672 + ECOLIN_RS23470 0.73 +0.2
4,729,696 + ECOLIN_RS23470 0.77 +0.2
4,729,800 - +3.8

Or see this region's nucleotide sequence