Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20490

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20480 and ECOLIN_RS20485 overlap by 8 nucleotidesECOLIN_RS20485 and ECOLIN_RS20490 overlap by 7 nucleotidesECOLIN_RS20490 and ECOLIN_RS20495 are separated by 46 nucleotides ECOLIN_RS20480: ECOLIN_RS20480 - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD, at 4,120,386 to 4,121,048 _RS20480 ECOLIN_RS20485: ECOLIN_RS20485 - L-ribulose-5-phosphate 3-epimerase, at 4,121,041 to 4,121,901 _RS20485 ECOLIN_RS20490: ECOLIN_RS20490 - L-ribulose-5-phosphate 4-epimerase, at 4,121,895 to 4,122,590 _RS20490 ECOLIN_RS20495: ECOLIN_RS20495 - aldehyde dehydrogenase AldB, at 4,122,637 to 4,124,175 _RS20495 Position (kb) 4121 4122 4123Strain fitness (log2 ratio) -1 0 1at 4121.046 kb on + strandat 4121.065 kb on + strandat 4121.244 kb on + strand, within ECOLIN_RS20485at 4121.244 kb on + strand, within ECOLIN_RS20485at 4121.370 kb on + strand, within ECOLIN_RS20485at 4121.371 kb on - strand, within ECOLIN_RS20485at 4121.373 kb on + strand, within ECOLIN_RS20485at 4121.374 kb on - strand, within ECOLIN_RS20485at 4121.390 kb on - strand, within ECOLIN_RS20485at 4121.398 kb on + strand, within ECOLIN_RS20485

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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4,121,046 + +0.2
4,121,065 + -0.2
4,121,244 + ECOLIN_RS20485 0.24 -0.1
4,121,244 + ECOLIN_RS20485 0.24 -0.4
4,121,370 + ECOLIN_RS20485 0.38 -0.1
4,121,371 - ECOLIN_RS20485 0.38 +0.3
4,121,373 + ECOLIN_RS20485 0.39 +0.9
4,121,374 - ECOLIN_RS20485 0.39 -0.1
4,121,390 - ECOLIN_RS20485 0.41 +0.6
4,121,398 + ECOLIN_RS20485 0.41 +0.2

Or see this region's nucleotide sequence