Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS18870

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS18860 and ECOLIN_RS18865 are separated by 129 nucleotidesECOLIN_RS18865 and ECOLIN_RS18870 are separated by 14 nucleotidesECOLIN_RS18870 and ECOLIN_RS18875 are separated by 51 nucleotides ECOLIN_RS18860: ECOLIN_RS18860 - DNA-protecting protein DprA, at 3,793,189 to 3,794,313 _RS18860 ECOLIN_RS18865: ECOLIN_RS18865 - peptide deformylase, at 3,794,443 to 3,794,952 _RS18865 ECOLIN_RS18870: ECOLIN_RS18870 - methionyl-tRNA formyltransferase, at 3,794,967 to 3,795,914 _RS18870 ECOLIN_RS18875: ECOLIN_RS18875 - 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB, at 3,795,966 to 3,797,255 _RS18875 Position (kb) 3794 3795 3796Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3794.093 kb on - strand, within ECOLIN_RS18860at 3794.093 kb on - strand, within ECOLIN_RS18860at 3794.181 kb on + strand, within ECOLIN_RS18860at 3794.181 kb on + strand, within ECOLIN_RS18860at 3794.182 kb on - strand, within ECOLIN_RS18860at 3794.201 kb on - strandat 3794.215 kb on - strandat 3794.254 kb on - strandat 3794.264 kb on + strandat 3794.264 kb on + strandat 3794.281 kb on - strandat 3794.330 kb on + strandat 3794.340 kb on + strandat 3794.397 kb on + strandat 3794.406 kb on + strandat 3794.442 kb on + strandat 3794.878 kb on + strand, within ECOLIN_RS18865at 3795.953 kb on + strandat 3796.754 kb on + strand, within ECOLIN_RS18875

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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3,794,093 - ECOLIN_RS18860 0.80 +0.4
3,794,093 - ECOLIN_RS18860 0.80 -0.1
3,794,181 + ECOLIN_RS18860 0.88 -0.5
3,794,181 + ECOLIN_RS18860 0.88 +0.2
3,794,182 - ECOLIN_RS18860 0.88 -0.2
3,794,201 - +0.2
3,794,215 - -1.4
3,794,254 - -0.4
3,794,264 + +0.9
3,794,264 + -2.6
3,794,281 - +0.1
3,794,330 + -0.4
3,794,340 + -0.1
3,794,397 + -0.7
3,794,406 + -0.2
3,794,442 + -0.8
3,794,878 + ECOLIN_RS18865 0.85 +4.5
3,795,953 + -0.6
3,796,754 + ECOLIN_RS18875 0.61 +0.5

Or see this region's nucleotide sequence