Experiment: JK16
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS13425 and ECOLIN_RS13430 are separated by 155 nucleotides ECOLIN_RS13430 and ECOLIN_RS13435 are separated by 82 nucleotides ECOLIN_RS13435 and ECOLIN_RS13440 overlap by 1 nucleotides
ECOLIN_RS13425: ECOLIN_RS13425 - acetyl-CoA carboxylase, carboxyltransferase subunit beta, at 2,686,873 to 2,687,787
_RS13425
ECOLIN_RS13430: ECOLIN_RS13430 - DedA family protein, at 2,687,943 to 2,688,602
_RS13430
ECOLIN_RS13435: ECOLIN_RS13435 - tRNA pseudouridine(38-40) synthase TruA, at 2,688,685 to 2,689,497
_RS13435
ECOLIN_RS13440: ECOLIN_RS13440 - aspartate-semialdehyde dehydrogenase, at 2,689,497 to 2,690,510
_RS13440
Position (kb)
2687
2688
2689 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2688.039 kb on - strand, within ECOLIN_RS13430 at 2688.334 kb on + strand, within ECOLIN_RS13430 at 2688.645 kb on - strand at 2688.898 kb on + strand, within ECOLIN_RS13435 at 2689.130 kb on + strand, within ECOLIN_RS13435 at 2689.226 kb on - strand, within ECOLIN_RS13435 at 2689.237 kb on + strand, within ECOLIN_RS13435 at 2689.499 kb on - strand at 2689.499 kb on - strand at 2689.499 kb on - strand at 2689.508 kb on - strand at 2689.537 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JK16 remove 2,688,039 - ECOLIN_RS13430 0.15 +0.1 2,688,334 + ECOLIN_RS13430 0.59 -0.3 2,688,645 - -0.3 2,688,898 + ECOLIN_RS13435 0.26 -0.5 2,689,130 + ECOLIN_RS13435 0.55 -0.1 2,689,226 - ECOLIN_RS13435 0.67 -2.9 2,689,237 + ECOLIN_RS13435 0.68 -3.6 2,689,499 - +0.1 2,689,499 - -0.7 2,689,499 - +2.3 2,689,508 - -0.5 2,689,537 - -0.7
Or see this region's nucleotide sequence