Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS13430

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS13425 and ECOLIN_RS13430 are separated by 155 nucleotidesECOLIN_RS13430 and ECOLIN_RS13435 are separated by 82 nucleotidesECOLIN_RS13435 and ECOLIN_RS13440 overlap by 1 nucleotides ECOLIN_RS13425: ECOLIN_RS13425 - acetyl-CoA carboxylase, carboxyltransferase subunit beta, at 2,686,873 to 2,687,787 _RS13425 ECOLIN_RS13430: ECOLIN_RS13430 - DedA family protein, at 2,687,943 to 2,688,602 _RS13430 ECOLIN_RS13435: ECOLIN_RS13435 - tRNA pseudouridine(38-40) synthase TruA, at 2,688,685 to 2,689,497 _RS13435 ECOLIN_RS13440: ECOLIN_RS13440 - aspartate-semialdehyde dehydrogenase, at 2,689,497 to 2,690,510 _RS13440 Position (kb) 2687 2688 2689Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2688.039 kb on - strand, within ECOLIN_RS13430at 2688.334 kb on + strand, within ECOLIN_RS13430at 2688.645 kb on - strandat 2688.898 kb on + strand, within ECOLIN_RS13435at 2689.130 kb on + strand, within ECOLIN_RS13435at 2689.226 kb on - strand, within ECOLIN_RS13435at 2689.237 kb on + strand, within ECOLIN_RS13435at 2689.499 kb on - strandat 2689.499 kb on - strandat 2689.499 kb on - strandat 2689.508 kb on - strandat 2689.537 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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2,688,039 - ECOLIN_RS13430 0.15 +0.1
2,688,334 + ECOLIN_RS13430 0.59 -0.3
2,688,645 - -0.3
2,688,898 + ECOLIN_RS13435 0.26 -0.5
2,689,130 + ECOLIN_RS13435 0.55 -0.1
2,689,226 - ECOLIN_RS13435 0.67 -2.9
2,689,237 + ECOLIN_RS13435 0.68 -3.6
2,689,499 - +0.1
2,689,499 - -0.7
2,689,499 - +2.3
2,689,508 - -0.5
2,689,537 - -0.7

Or see this region's nucleotide sequence