Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS10055

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS10045 and ECOLIN_RS10050 are separated by 39 nucleotidesECOLIN_RS10050 and ECOLIN_RS10055 are separated by 57 nucleotidesECOLIN_RS10055 and ECOLIN_RS10060 are separated by 131 nucleotidesECOLIN_RS10060 and ECOLIN_RS10070 are separated by 421 nucleotides ECOLIN_RS10045: ECOLIN_RS10045 - Slp family lipoprotein YeaY, at 1,973,936 to 1,974,517 _RS10045 ECOLIN_RS10050: ECOLIN_RS10050 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB, at 1,974,557 to 1,975,252 _RS10050 ECOLIN_RS10055: ECOLIN_RS10055 - ATP-dependent DNA helicase, at 1,975,310 to 1,977,220 _RS10055 ECOLIN_RS10060: ECOLIN_RS10060 - RidA family protein, at 1,977,352 to 1,977,696 _RS10060 ECOLIN_RS10070: ECOLIN_RS10070 - DUF1889 family protein, at 1,978,118 to 1,978,417 _RS10070 Position (kb) 1975 1976 1977 1978Strain fitness (log2 ratio) -2 -1 0 1at 1974.445 kb on + strand, within ECOLIN_RS10045at 1975.267 kb on - strandat 1975.939 kb on - strand, within ECOLIN_RS10055

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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1,974,445 + ECOLIN_RS10045 0.87 -1.0
1,975,267 - -1.8
1,975,939 - ECOLIN_RS10055 0.33 +0.2

Or see this region's nucleotide sequence