Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS00175

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS00160 and ECOLIN_RS00175 are separated by 455 nucleotidesECOLIN_RS00175 and ECOLIN_RS00180 are separated by 17 nucleotides ECOLIN_RS00160: ECOLIN_RS00160 - 4-hydroxy-tetrahydrodipicolinate reductase, at 30,332 to 31,153 _RS00160 ECOLIN_RS00175: ECOLIN_RS00175 - glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit, at 31,609 to 32,757 _RS00175 ECOLIN_RS00180: ECOLIN_RS00180 - carbamoyl-phosphate synthase large subunit, at 32,775 to 35,996 _RS00180 Position (kb) 31 32 33Strain fitness (log2 ratio) -1 0 1at 31.773 kb on + strand, within ECOLIN_RS00175at 31.774 kb on - strand, within ECOLIN_RS00175at 31.777 kb on + strand, within ECOLIN_RS00175at 31.777 kb on + strand, within ECOLIN_RS00175at 32.111 kb on - strand, within ECOLIN_RS00175at 32.112 kb on + strand, within ECOLIN_RS00175at 32.112 kb on + strand, within ECOLIN_RS00175at 32.596 kb on - strand, within ECOLIN_RS00175at 32.742 kb on + strandat 32.756 kb on - strandat 32.765 kb on + strandat 32.933 kb on + strandat 32.967 kb on + strandat 33.515 kb on + strand, within ECOLIN_RS00180

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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31,773 + ECOLIN_RS00175 0.14 +0.2
31,774 - ECOLIN_RS00175 0.14 +0.2
31,777 + ECOLIN_RS00175 0.15 +0.2
31,777 + ECOLIN_RS00175 0.15 +0.1
32,111 - ECOLIN_RS00175 0.44 -1.2
32,112 + ECOLIN_RS00175 0.44 +0.9
32,112 + ECOLIN_RS00175 0.44 +0.4
32,596 - ECOLIN_RS00175 0.86 +0.8
32,742 + -0.5
32,756 - -0.1
32,765 + +0.2
32,933 + -0.3
32,967 + -0.4
33,515 + ECOLIN_RS00180 0.23 -0.1

Or see this region's nucleotide sequence