Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20680

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20670 and ECOLIN_RS20675 are separated by 25 nucleotidesECOLIN_RS20675 and ECOLIN_RS20680 are separated by 9 nucleotidesECOLIN_RS20680 and ECOLIN_RS20685 are separated by 16 nucleotides ECOLIN_RS20670: ECOLIN_RS20670 - UDP-galactose--(galactosyl) LPS alpha1,2-galactosyltransferase, at 4,165,689 to 4,166,717 _RS20670 ECOLIN_RS20675: ECOLIN_RS20675 - lipopolysaccharide core heptose(II) kinase RfaY, at 4,166,743 to 4,167,435 _RS20675 ECOLIN_RS20680: ECOLIN_RS20680 - glycosyltransferase, at 4,167,445 to 4,168,440 _RS20680 ECOLIN_RS20685: ECOLIN_RS20685 - lipopolysaccharide 3-alpha-galactosyltransferase, at 4,168,457 to 4,169,473 _RS20685 Position (kb) 4167 4168 4169Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4166.709 kb on + strandat 4167.194 kb on + strand, within ECOLIN_RS20675at 4167.422 kb on - strandat 4167.741 kb on + strand, within ECOLIN_RS20680at 4167.903 kb on - strand, within ECOLIN_RS20680at 4167.975 kb on + strand, within ECOLIN_RS20680at 4168.015 kb on - strand, within ECOLIN_RS20680at 4168.021 kb on - strand, within ECOLIN_RS20680at 4168.128 kb on - strand, within ECOLIN_RS20680at 4168.128 kb on - strand, within ECOLIN_RS20680at 4168.338 kb on - strand, within ECOLIN_RS20680at 4168.489 kb on + strandat 4169.040 kb on - strand, within ECOLIN_RS20685at 4169.040 kb on - strand, within ECOLIN_RS20685at 4169.126 kb on + strand, within ECOLIN_RS20685at 4169.140 kb on - strand, within ECOLIN_RS20685at 4169.150 kb on - strand, within ECOLIN_RS20685at 4169.219 kb on - strand, within ECOLIN_RS20685at 4169.219 kb on - strand, within ECOLIN_RS20685at 4169.223 kb on + strand, within ECOLIN_RS20685at 4169.311 kb on + strand, within ECOLIN_RS20685at 4169.312 kb on - strand, within ECOLIN_RS20685

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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4,166,709 + -2.0
4,167,194 + ECOLIN_RS20675 0.65 -2.8
4,167,422 - -1.1
4,167,741 + ECOLIN_RS20680 0.30 -2.9
4,167,903 - ECOLIN_RS20680 0.46 -1.0
4,167,975 + ECOLIN_RS20680 0.53 -4.2
4,168,015 - ECOLIN_RS20680 0.57 -0.3
4,168,021 - ECOLIN_RS20680 0.58 -0.7
4,168,128 - ECOLIN_RS20680 0.69 -0.2
4,168,128 - ECOLIN_RS20680 0.69 -0.5
4,168,338 - ECOLIN_RS20680 0.90 -0.9
4,168,489 + -1.9
4,169,040 - ECOLIN_RS20685 0.57 +1.1
4,169,040 - ECOLIN_RS20685 0.57 -0.8
4,169,126 + ECOLIN_RS20685 0.66 -1.0
4,169,140 - ECOLIN_RS20685 0.67 -0.6
4,169,150 - ECOLIN_RS20685 0.68 -2.2
4,169,219 - ECOLIN_RS20685 0.75 -0.7
4,169,219 - ECOLIN_RS20685 0.75 -0.7
4,169,223 + ECOLIN_RS20685 0.75 -1.1
4,169,311 + ECOLIN_RS20685 0.84 -0.7
4,169,312 - ECOLIN_RS20685 0.84 -1.4

Or see this region's nucleotide sequence