Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS13225

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS13215 and ECOLIN_RS13220 are separated by 102 nucleotidesECOLIN_RS13220 and ECOLIN_RS13225 are separated by 66 nucleotidesECOLIN_RS13225 and ECOLIN_RS13230 are separated by 6 nucleotides ECOLIN_RS13215: ECOLIN_RS13215 - M28 family metallopeptidase, at 2,642,973 to 2,643,935 _RS13215 ECOLIN_RS13220: ECOLIN_RS13220 - YfbM family protein, at 2,644,038 to 2,644,541 _RS13220 ECOLIN_RS13225: ECOLIN_RS13225 - NADH-quinone oxidoreductase subunit NuoN, at 2,644,608 to 2,646,065 _RS13225 ECOLIN_RS13230: ECOLIN_RS13230 - NADH-quinone oxidoreductase subunit M, at 2,646,072 to 2,647,601 _RS13230 Position (kb) 2644 2645 2646 2647Strain fitness (log2 ratio) -3 -2 -1 0 1at 2644.799 kb on - strand, within ECOLIN_RS13225at 2644.877 kb on - strand, within ECOLIN_RS13225at 2645.160 kb on - strand, within ECOLIN_RS13225at 2645.160 kb on - strand, within ECOLIN_RS13225at 2645.160 kb on - strand, within ECOLIN_RS13225at 2645.374 kb on + strand, within ECOLIN_RS13225at 2645.374 kb on + strand, within ECOLIN_RS13225at 2645.377 kb on - strand, within ECOLIN_RS13225at 2645.533 kb on + strand, within ECOLIN_RS13225at 2645.534 kb on - strand, within ECOLIN_RS13225at 2645.736 kb on + strand, within ECOLIN_RS13225at 2645.737 kb on - strand, within ECOLIN_RS13225at 2645.737 kb on - strand, within ECOLIN_RS13225at 2645.840 kb on - strand, within ECOLIN_RS13225at 2646.057 kb on + strandat 2646.058 kb on - strandat 2646.084 kb on - strandat 2646.084 kb on - strandat 2646.087 kb on - strandat 2646.506 kb on + strand, within ECOLIN_RS13230at 2646.611 kb on - strand, within ECOLIN_RS13230at 2646.703 kb on + strand, within ECOLIN_RS13230at 2646.982 kb on + strand, within ECOLIN_RS13230at 2647.012 kb on + strand, within ECOLIN_RS13230at 2647.013 kb on - strand, within ECOLIN_RS13230at 2647.013 kb on - strand, within ECOLIN_RS13230

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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2,644,799 - ECOLIN_RS13225 0.13 -1.8
2,644,877 - ECOLIN_RS13225 0.18 -1.0
2,645,160 - ECOLIN_RS13225 0.38 -0.0
2,645,160 - ECOLIN_RS13225 0.38 -0.7
2,645,160 - ECOLIN_RS13225 0.38 -1.4
2,645,374 + ECOLIN_RS13225 0.53 -0.8
2,645,374 + ECOLIN_RS13225 0.53 -0.2
2,645,377 - ECOLIN_RS13225 0.53 -1.7
2,645,533 + ECOLIN_RS13225 0.63 -2.4
2,645,534 - ECOLIN_RS13225 0.64 -0.6
2,645,736 + ECOLIN_RS13225 0.77 -1.3
2,645,737 - ECOLIN_RS13225 0.77 -1.3
2,645,737 - ECOLIN_RS13225 0.77 -1.2
2,645,840 - ECOLIN_RS13225 0.84 -0.4
2,646,057 + -0.1
2,646,058 - -0.8
2,646,084 - -0.6
2,646,084 - -0.9
2,646,087 - -0.2
2,646,506 + ECOLIN_RS13230 0.28 -2.1
2,646,611 - ECOLIN_RS13230 0.35 -1.3
2,646,703 + ECOLIN_RS13230 0.41 +0.4
2,646,982 + ECOLIN_RS13230 0.59 -0.5
2,647,012 + ECOLIN_RS13230 0.61 -2.8
2,647,013 - ECOLIN_RS13230 0.62 -1.2
2,647,013 - ECOLIN_RS13230 0.62 +0.7

Or see this region's nucleotide sequence