Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12190

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12180 and ECOLIN_RS12185 overlap by 4 nucleotidesECOLIN_RS12185 and ECOLIN_RS12190 overlap by 4 nucleotidesECOLIN_RS12190 and ECOLIN_RS31520 are separated by 217 nucleotidesECOLIN_RS31520 and ECOLIN_RS31525 are separated by 271 nucleotidesECOLIN_RS31525 and ECOLIN_RS12195 are separated by 278 nucleotides ECOLIN_RS12180: ECOLIN_RS12180 - protein kinase YegI, at 2,409,543 to 2,411,483 _RS12180 ECOLIN_RS12185: ECOLIN_RS12185 - protein-serine/threonine phosphatase PphC, at 2,411,480 to 2,412,241 _RS12185 ECOLIN_RS12190: ECOLIN_RS12190 - VWA domain-containing protein, at 2,412,238 to 2,412,897 _RS12190 ECOLIN_RS31520: ECOLIN_RS31520 - type I toxin-antitoxin system Ibs family toxin, at 2,413,115 to 2,413,171 _RS31520 ECOLIN_RS31525: ECOLIN_RS31525 - type I toxin-antitoxin system Ibs family toxin, at 2,413,443 to 2,413,499 _RS31525 ECOLIN_RS12195: ECOLIN_RS12195 - multidrug efflux RND transporter subunit MdtA, at 2,413,778 to 2,415,025 _RS12195 Position (kb) 2412 2413Strain fitness (log2 ratio) -1 0 1at 2412.040 kb on - strand, within ECOLIN_RS12185at 2412.920 kb on + strandat 2413.116 kb on + strandat 2413.444 kb on + strandat 2413.444 kb on + strandat 2413.467 kb on + strand, within ECOLIN_RS31525

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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2,412,040 - ECOLIN_RS12185 0.73 +0.8
2,412,920 + +0.0
2,413,116 + -0.8
2,413,444 + +0.4
2,413,444 + +0.3
2,413,467 + ECOLIN_RS31525 0.42 +0.3

Or see this region's nucleotide sequence