Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12115

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12110 and ECOLIN_RS12115 overlap by 26 nucleotidesECOLIN_RS12115 and ECOLIN_RS12120 overlap by 4 nucleotidesECOLIN_RS12120 and ECOLIN_RS12125 are separated by 2 nucleotides ECOLIN_RS12110: ECOLIN_RS12110 - colanic acid polymerase WcaD, at 2,390,370 to 2,391,587 _RS12110 ECOLIN_RS12115: ECOLIN_RS12115 - colanic acid biosynthesis glycosyltransferase WcaC, at 2,391,562 to 2,392,779 _RS12115 ECOLIN_RS12120: ECOLIN_RS12120 - colanic acid biosynthesis acetyltransferase WcaB, at 2,392,776 to 2,393,264 _RS12120 ECOLIN_RS12125: ECOLIN_RS12125 - colanic acid biosynthesis glycosyltransferase WcaA, at 2,393,267 to 2,394,106 _RS12125 Position (kb) 2391 2392 2393Strain fitness (log2 ratio) -1 0 1at 2391.317 kb on + strand, within ECOLIN_RS12110at 2391.318 kb on - strand, within ECOLIN_RS12110at 2391.560 kb on - strandat 2392.126 kb on - strand, within ECOLIN_RS12115at 2392.295 kb on - strand, within ECOLIN_RS12115

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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2,391,317 + ECOLIN_RS12110 0.78 -0.0
2,391,318 - ECOLIN_RS12110 0.78 -0.2
2,391,560 - +0.6
2,392,126 - ECOLIN_RS12115 0.46 +0.5
2,392,295 - ECOLIN_RS12115 0.60 -0.3

Or see this region's nucleotide sequence