Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20455

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20445 and ECOLIN_RS20450 are separated by 11 nucleotidesECOLIN_RS20450 and ECOLIN_RS20455 are separated by 22 nucleotidesECOLIN_RS20455 and ECOLIN_RS20460 are separated by 113 nucleotidesECOLIN_RS20460 and ECOLIN_RS20465 are separated by 2 nucleotides ECOLIN_RS20445: ECOLIN_RS20445 - 3-dehydro-L-gulonate 2-dehydrogenase, at 4,113,612 to 4,114,610 _RS20445 ECOLIN_RS20450: ECOLIN_RS20450 - YhcH/YjgK/YiaL family protein, at 4,114,622 to 4,115,086 _RS20450 ECOLIN_RS20455: ECOLIN_RS20455 - DUF4862 family protein, at 4,115,109 to 4,116,029 _RS20455 ECOLIN_RS20460: ECOLIN_RS20460 - 2,3-diketo-L-gulonate TRAP transporter small permease YiaM, at 4,116,143 to 4,116,610 _RS20460 ECOLIN_RS20465: ECOLIN_RS20465 - 2,3-diketo-L-gulonate TRAP transporter large permease YiaN, at 4,116,613 to 4,117,890 _RS20465 Position (kb) 4115 4116 4117Strain fitness (log2 ratio) -2 -1 0 1at 4114.159 kb on + strand, within ECOLIN_RS20445at 4114.160 kb on - strand, within ECOLIN_RS20445at 4114.195 kb on + strand, within ECOLIN_RS20445at 4114.314 kb on - strand, within ECOLIN_RS20445at 4114.470 kb on + strand, within ECOLIN_RS20445at 4114.470 kb on + strand, within ECOLIN_RS20445at 4114.471 kb on - strand, within ECOLIN_RS20445at 4114.471 kb on - strand, within ECOLIN_RS20445at 4114.532 kb on + strandat 4114.533 kb on - strandat 4114.565 kb on + strandat 4114.616 kb on + strandat 4114.823 kb on + strand, within ECOLIN_RS20450at 4114.912 kb on - strand, within ECOLIN_RS20450at 4114.978 kb on + strand, within ECOLIN_RS20450at 4114.978 kb on + strand, within ECOLIN_RS20450at 4115.008 kb on - strand, within ECOLIN_RS20450at 4115.024 kb on - strand, within ECOLIN_RS20450at 4115.132 kb on + strandat 4115.133 kb on - strandat 4115.133 kb on - strandat 4115.210 kb on - strand, within ECOLIN_RS20455at 4115.294 kb on + strand, within ECOLIN_RS20455at 4115.416 kb on - strand, within ECOLIN_RS20455at 4115.436 kb on - strand, within ECOLIN_RS20455at 4115.833 kb on + strand, within ECOLIN_RS20455at 4115.833 kb on + strand, within ECOLIN_RS20455at 4115.954 kb on - strandat 4116.397 kb on + strand, within ECOLIN_RS20460at 4116.502 kb on - strand, within ECOLIN_RS20460at 4116.561 kb on + strand, within ECOLIN_RS20460at 4116.561 kb on + strand, within ECOLIN_RS20460at 4116.561 kb on + strand, within ECOLIN_RS20460at 4116.562 kb on - strand, within ECOLIN_RS20460at 4116.624 kb on + strandat 4116.625 kb on - strandat 4116.684 kb on - strandat 4116.755 kb on + strand, within ECOLIN_RS20465at 4116.755 kb on + strand, within ECOLIN_RS20465at 4116.801 kb on - strand, within ECOLIN_RS20465at 4116.843 kb on + strand, within ECOLIN_RS20465

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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4,114,159 + ECOLIN_RS20445 0.55 +0.6
4,114,160 - ECOLIN_RS20445 0.55 +0.0
4,114,195 + ECOLIN_RS20445 0.58 +1.2
4,114,314 - ECOLIN_RS20445 0.70 -0.3
4,114,470 + ECOLIN_RS20445 0.86 +0.5
4,114,470 + ECOLIN_RS20445 0.86 +0.1
4,114,471 - ECOLIN_RS20445 0.86 +0.2
4,114,471 - ECOLIN_RS20445 0.86 +0.2
4,114,532 + +0.4
4,114,533 - +0.7
4,114,565 + -0.1
4,114,616 + +0.2
4,114,823 + ECOLIN_RS20450 0.43 +0.1
4,114,912 - ECOLIN_RS20450 0.62 -0.1
4,114,978 + ECOLIN_RS20450 0.77 -1.7
4,114,978 + ECOLIN_RS20450 0.77 -0.5
4,115,008 - ECOLIN_RS20450 0.83 +0.1
4,115,024 - ECOLIN_RS20450 0.86 -0.2
4,115,132 + -0.2
4,115,133 - -0.8
4,115,133 - +0.9
4,115,210 - ECOLIN_RS20455 0.11 -0.2
4,115,294 + ECOLIN_RS20455 0.20 -0.2
4,115,416 - ECOLIN_RS20455 0.33 +0.1
4,115,436 - ECOLIN_RS20455 0.36 -0.0
4,115,833 + ECOLIN_RS20455 0.79 -0.3
4,115,833 + ECOLIN_RS20455 0.79 +0.2
4,115,954 - +0.6
4,116,397 + ECOLIN_RS20460 0.54 +0.3
4,116,502 - ECOLIN_RS20460 0.77 -0.5
4,116,561 + ECOLIN_RS20460 0.89 -0.2
4,116,561 + ECOLIN_RS20460 0.89 -0.1
4,116,561 + ECOLIN_RS20460 0.89 +0.8
4,116,562 - ECOLIN_RS20460 0.90 -0.1
4,116,624 + +0.8
4,116,625 - -2.1
4,116,684 - +1.0
4,116,755 + ECOLIN_RS20465 0.11 +0.3
4,116,755 + ECOLIN_RS20465 0.11 -0.0
4,116,801 - ECOLIN_RS20465 0.15 -2.3
4,116,843 + ECOLIN_RS20465 0.18 -2.5

Or see this region's nucleotide sequence