Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS16755

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS16745 and ECOLIN_RS16750 are separated by 48 nucleotidesECOLIN_RS16750 and ECOLIN_RS16755 are separated by 33 nucleotidesECOLIN_RS16755 and ECOLIN_RS32990 are separated by 117 nucleotidesECOLIN_RS32990 and ECOLIN_RS16770 are separated by 145 nucleotides ECOLIN_RS16745: ECOLIN_RS16745 - MFS transporter, at 3,383,687 to 3,385,159 _RS16745 ECOLIN_RS16750: ECOLIN_RS16750 - N-acetylmannosamine kinase, at 3,385,208 to 3,386,083 _RS16750 ECOLIN_RS16755: ECOLIN_RS16755 - N-acetylneuraminate lyase, at 3,386,117 to 3,387,034 _RS16755 ECOLIN_RS32990: ECOLIN_RS32990 - transposase domain-containing protein, at 3,387,152 to 3,387,553 _RS32990 ECOLIN_RS16770: ECOLIN_RS16770 - transposase, at 3,387,699 to 3,388,124 _RS16770 Position (kb) 3386 3387 3388Strain fitness (log2 ratio) -1 0 1at 3386.908 kb on - strand, within ECOLIN_RS16755at 3386.908 kb on - strand, within ECOLIN_RS16755at 3386.935 kb on + strand, within ECOLIN_RS16755at 3387.554 kb on + strandat 3387.555 kb on - strandat 3387.586 kb on - strandat 3387.604 kb on + strandat 3387.662 kb on - strandat 3387.684 kb on + strandat 3387.752 kb on + strand, within ECOLIN_RS16770at 3387.988 kb on + strand, within ECOLIN_RS16770

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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3,386,908 - ECOLIN_RS16755 0.86 +0.1
3,386,908 - ECOLIN_RS16755 0.86 -0.7
3,386,935 + ECOLIN_RS16755 0.89 +0.4
3,387,554 + -0.4
3,387,555 - +0.0
3,387,586 - -1.6
3,387,604 + -0.1
3,387,662 - +0.1
3,387,684 + -0.2
3,387,752 + ECOLIN_RS16770 0.12 -0.6
3,387,988 + ECOLIN_RS16770 0.68 +0.4

Or see this region's nucleotide sequence