Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS13555

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS13550 and ECOLIN_RS13555 overlap by 1 nucleotidesECOLIN_RS13555 and ECOLIN_RS13560 are separated by 179 nucleotidesECOLIN_RS13560 and ECOLIN_RS13565 are separated by 371 nucleotides ECOLIN_RS13550: ECOLIN_RS13550 - fatty acid oxidation complex subunit alpha FadJ, at 2,711,039 to 2,713,183 _RS13550 ECOLIN_RS13555: ECOLIN_RS13555 - acetyl-CoA C-acyltransferase FadI, at 2,713,183 to 2,714,493 _RS13555 ECOLIN_RS13560: ECOLIN_RS13560 - YfcZ/YiiS family protein, at 2,714,673 to 2,714,957 _RS13560 ECOLIN_RS13565: ECOLIN_RS13565 - long-chain fatty acid transporter FadL, at 2,715,329 to 2,716,669 _RS13565 Position (kb) 2713 2714 2715Strain fitness (log2 ratio) -1 0 1 2 3 4at 2712.453 kb on + strand, within ECOLIN_RS13550at 2713.567 kb on + strand, within ECOLIN_RS13555at 2713.792 kb on - strand, within ECOLIN_RS13555at 2713.982 kb on - strand, within ECOLIN_RS13555at 2714.657 kb on - strandat 2714.946 kb on - strandat 2714.952 kb on - strandat 2714.952 kb on - strandat 2715.293 kb on + strandat 2715.308 kb on + strandat 2715.382 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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2,712,453 + ECOLIN_RS13550 0.66 +4.3
2,713,567 + ECOLIN_RS13555 0.29 -0.6
2,713,792 - ECOLIN_RS13555 0.46 -0.4
2,713,982 - ECOLIN_RS13555 0.61 -0.0
2,714,657 - +0.3
2,714,946 - -0.1
2,714,952 - +0.6
2,714,952 - -0.2
2,715,293 + +1.0
2,715,308 + +4.4
2,715,382 + -0.6

Or see this region's nucleotide sequence