Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS09645

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS09635 and ECOLIN_RS09640 are separated by 146 nucleotidesECOLIN_RS09640 and ECOLIN_RS09645 are separated by 163 nucleotidesECOLIN_RS09645 and ECOLIN_RS09650 are separated by 132 nucleotides ECOLIN_RS09635: ECOLIN_RS09635 - YniB family protein, at 1,896,105 to 1,896,641 _RS09635 ECOLIN_RS09640: ECOLIN_RS09640 - hexitol phosphatase HxpB, at 1,896,788 to 1,897,456 _RS09640 ECOLIN_RS09645: ECOLIN_RS09645 - metal-dependent hydrolase, at 1,897,620 to 1,898,210 _RS09645 ECOLIN_RS09650: ECOLIN_RS09650 - cystine/sulfocysteine:cation symporter, at 1,898,343 to 1,899,734 _RS09650 Position (kb) 1897 1898 1899Strain fitness (log2 ratio) -1 0 1at 1898.296 kb on + strandat 1898.383 kb on + strandat 1898.493 kb on + strand, within ECOLIN_RS09650at 1898.537 kb on + strand, within ECOLIN_RS09650at 1898.798 kb on + strand, within ECOLIN_RS09650at 1898.802 kb on + strand, within ECOLIN_RS09650

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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1,898,296 + +0.3
1,898,383 + +0.8
1,898,493 + ECOLIN_RS09650 0.11 +1.3
1,898,537 + ECOLIN_RS09650 0.14 +0.2
1,898,798 + ECOLIN_RS09650 0.33 +0.2
1,898,802 + ECOLIN_RS09650 0.33 -1.0

Or see this region's nucleotide sequence