Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS04245

Experiment: Bas05

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS04235 and ECOLIN_RS04240 are separated by 2 nucleotidesECOLIN_RS04240 and ECOLIN_RS04245 are separated by 228 nucleotidesECOLIN_RS04245 and ECOLIN_RS04250 are separated by 232 nucleotidesECOLIN_RS04250 and ECOLIN_RS04255 are separated by 103 nucleotides ECOLIN_RS04235: ECOLIN_RS04235 - secretion protein HlyD, at 856,705 to 857,700 _RS04235 ECOLIN_RS04240: ECOLIN_RS04240 - DNA-binding transcriptional regulator CecR, at 857,703 to 858,374 _RS04240 ECOLIN_RS04245: ECOLIN_RS04245 - ATP-dependent RNA helicase RhlE, at 858,603 to 859,964 _RS04245 ECOLIN_RS04250: ECOLIN_RS04250 - NADAR domain-containing protein, at 860,197 to 860,397 _RS04250 ECOLIN_RS04255: ECOLIN_RS04255 - ATP-dependent DNA helicase DinG, at 860,501 to 862,651 _RS04255 Position (kb) 858 859 860Strain fitness (log2 ratio) -1 0 1at 857.663 kb on - strandat 857.666 kb on - strandat 857.666 kb on - strandat 858.227 kb on - strand, within ECOLIN_RS04240at 858.895 kb on - strand, within ECOLIN_RS04245at 859.185 kb on - strand, within ECOLIN_RS04245at 859.316 kb on + strand, within ECOLIN_RS04245at 859.400 kb on + strand, within ECOLIN_RS04245at 859.587 kb on + strand, within ECOLIN_RS04245at 859.587 kb on + strand, within ECOLIN_RS04245at 859.588 kb on - strand, within ECOLIN_RS04245

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas05
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857,663 - -0.4
857,666 - -0.9
857,666 - +0.3
858,227 - ECOLIN_RS04240 0.78 -1.3
858,895 - ECOLIN_RS04245 0.21 +0.2
859,185 - ECOLIN_RS04245 0.43 -0.7
859,316 + ECOLIN_RS04245 0.52 +1.6
859,400 + ECOLIN_RS04245 0.59 +0.6
859,587 + ECOLIN_RS04245 0.72 +0.5
859,587 + ECOLIN_RS04245 0.72 +0.1
859,588 - ECOLIN_RS04245 0.72 -0.4

Or see this region's nucleotide sequence