Experiment: MoYLS4 with DMSO 7.5%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prfB and DVU1862 overlap by 20 nucleotides DVU1862 and DVU1863 overlap by 4 nucleotides DVU1863 and ihfB are separated by 74 nucleotides ihfB and DVU1865 overlap by 14 nucleotides DVU1865 and DVU1866 are separated by 51 nucleotides
DVU1861: prfB - peptide chain release factor 2, programmed frameshift (TIGR), at 1,928,351 to 1,929,467
prfB
DVU1862: DVU1862 - GGDEF domain protein (TIGR), at 1,929,448 to 1,930,443
DVU1862
DVU1863: DVU1863 - flagellar synthesis regulator FleN, putative (TIGR), at 1,930,440 to 1,931,258
DVU1863
DVU1864: ihfB - DNA-binding protein HU, beta subunit, putative (TIGR), at 1,931,333 to 1,931,614
ihfB
DVU1865: DVU1865 - hypothetical protein (TIGR), at 1,931,601 to 1,931,741
DVU1865
DVU1866: DVU1866 - hypothetical protein (TIGR), at 1,931,793 to 1,932,494
DVU1866
Position (kb)
1930
1931
1932 Strain fitness (log2 ratio)
-2
-1
0
1 at 1929.486 kb on - strand at 1929.486 kb on - strand at 1929.619 kb on + strand, within DVU1862 at 1929.649 kb on - strand, within DVU1862 at 1929.649 kb on - strand, within DVU1862 at 1929.685 kb on + strand, within DVU1862 at 1929.798 kb on + strand, within DVU1862 at 1929.943 kb on + strand, within DVU1862 at 1930.055 kb on + strand, within DVU1862 at 1930.101 kb on + strand, within DVU1862 at 1930.111 kb on + strand, within DVU1862 at 1930.138 kb on + strand, within DVU1862 at 1930.379 kb on - strand at 1930.387 kb on - strand at 1930.421 kb on + strand at 1930.421 kb on + strand at 1930.429 kb on - strand at 1930.467 kb on + strand at 1930.469 kb on - strand at 1930.499 kb on + strand at 1930.668 kb on - strand, within DVU1863 at 1930.673 kb on - strand, within DVU1863 at 1930.763 kb on - strand, within DVU1863 at 1930.779 kb on + strand, within DVU1863 at 1930.909 kb on + strand, within DVU1863 at 1930.927 kb on - strand, within DVU1863 at 1931.078 kb on + strand, within DVU1863 at 1931.090 kb on + strand, within DVU1863 at 1931.204 kb on - strand at 1931.252 kb on + strand at 1931.329 kb on - strand at 1931.350 kb on + strand at 1931.608 kb on + strand at 1931.729 kb on - strand at 1931.773 kb on + strand at 1931.864 kb on + strand, within DVU1866 at 1932.025 kb on + strand, within DVU1866 at 1932.151 kb on - strand, within DVU1866 at 1932.161 kb on - strand, within DVU1866 at 1932.174 kb on + strand, within DVU1866 at 1932.246 kb on + strand, within DVU1866
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with DMSO 7.5% remove 1,929,486 - -0.4 1,929,486 - -0.0 1,929,619 + DVU1862 0.17 -1.1 1,929,649 - DVU1862 0.20 -2.5 1,929,649 - DVU1862 0.20 -0.3 1,929,685 + DVU1862 0.24 -0.6 1,929,798 + DVU1862 0.35 -0.6 1,929,943 + DVU1862 0.50 -1.1 1,930,055 + DVU1862 0.61 -0.8 1,930,101 + DVU1862 0.66 -0.4 1,930,111 + DVU1862 0.67 -0.9 1,930,138 + DVU1862 0.69 -0.7 1,930,379 - -0.3 1,930,387 - -0.4 1,930,421 + +0.6 1,930,421 + -0.2 1,930,429 - -0.4 1,930,467 + -0.9 1,930,469 - -0.3 1,930,499 + -0.6 1,930,668 - DVU1863 0.28 -2.0 1,930,673 - DVU1863 0.28 -0.9 1,930,763 - DVU1863 0.39 -1.0 1,930,779 + DVU1863 0.41 -0.7 1,930,909 + DVU1863 0.57 -0.7 1,930,927 - DVU1863 0.59 -0.1 1,931,078 + DVU1863 0.78 -0.7 1,931,090 + DVU1863 0.79 -1.0 1,931,204 - +0.0 1,931,252 + +1.6 1,931,329 - +0.3 1,931,350 + -0.2 1,931,608 + -1.0 1,931,729 - -0.3 1,931,773 + -0.2 1,931,864 + DVU1866 0.10 +0.0 1,932,025 + DVU1866 0.33 +0.8 1,932,151 - DVU1866 0.51 -0.2 1,932,161 - DVU1866 0.52 -0.8 1,932,174 + DVU1866 0.54 -1.3 1,932,246 + DVU1866 0.65 -1.6
Or see this region's nucleotide sequence