Experiment: MoYLS4 with DMSO 7.5%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1674 and DVU1675 are separated by 58 nucleotides DVU1675 and tRNA-Leu are separated by 240 nucleotides tRNA-Leu and secG are separated by 12 nucleotides secG and tpiA are separated by 37 nucleotides
DVU1674: DVU1674 - transcriptional regulator, putative (TIGR), at 1,754,716 to 1,755,330
DVU1674
DVU1675: DVU1675 - hypothetical protein (TIGR), at 1,755,389 to 1,755,553
DVU1675
DVU_tRNA-Leu-4: tRNA-Leu - tRNA with anticodon GAG for Leu (VIMSS-AUTO), at 1,755,794 to 1,755,880
tRNA-Leu
DVU1676: secG - preprotein translocase, SecG subunit (TIGR), at 1,755,893 to 1,756,243
secG
DVU1677: tpiA - triosephosphate isomerase (TIGR), at 1,756,281 to 1,757,036
tpiA
Position (kb) 1755
1756 Strain fitness (log2 ratio) -2
-1
0
1 at 1754.881 kb on + strand, within DVU1674 at 1755.330 kb on - strand at 1755.564 kb on - strand at 1755.670 kb on - strand at 1755.675 kb on + strand at 1755.818 kb on - strand, within tRNA-Leu at 1755.843 kb on + strand, within tRNA-Leu at 1755.924 kb on + strand at 1755.932 kb on - strand, within secG at 1755.999 kb on + strand, within secG
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with DMSO 7.5% remove 1,754,881 + DVU1674 0.27 +0.2 1,755,330 - -0.4 1,755,564 - -0.3 1,755,670 - -0.5 1,755,675 + -0.1 1,755,818 - tRNA-Leu DVU_tRNA-Leu-4 0.28 -0.4 1,755,843 + tRNA-Leu DVU_tRNA-Leu-4 0.56 +0.9 1,755,924 + -2.0 1,755,932 - secG DVU1676 0.11 -0.3 1,755,999 + secG DVU1676 0.30 -0.1
Or see this region's nucleotide sequence