Experiment: MoYLS4 with DMSO 7.5%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysW and cysA overlap by 4 nucleotides cysA and acs are separated by 85 nucleotides acs and DVU0749 are separated by 49 nucleotides
DVU0746: cysW - ABC transporter, permease protein (TIGR), at 835,639 to 836,385
cysW
DVU0747: cysA - ABC transporter, ATP-binding protein (TIGR), at 836,382 to 837,020
cysA
DVU0748: acs - acetyl-CoA synthetase (TIGR), at 837,106 to 839,052
acs
DVU0749: DVU0749 - DNA-binding response regulator (TIGR), at 839,102 to 840,031
DVU0749
Position (kb)
837
838
839
840 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 836.143 kb on + strand, within cysW at 836.372 kb on + strand at 836.709 kb on + strand, within cysA at 836.751 kb on - strand, within cysA at 836.838 kb on + strand, within cysA at 836.843 kb on + strand, within cysA at 836.871 kb on + strand, within cysA at 836.953 kb on + strand, within cysA at 837.048 kb on - strand at 837.118 kb on - strand at 837.136 kb on - strand at 837.145 kb on + strand at 837.228 kb on - strand at 837.268 kb on - strand at 837.276 kb on + strand at 837.287 kb on + strand at 837.430 kb on - strand, within acs at 837.787 kb on + strand, within acs at 837.810 kb on + strand, within acs at 837.818 kb on - strand, within acs at 837.826 kb on + strand, within acs at 837.905 kb on + strand, within acs at 837.905 kb on + strand, within acs at 837.907 kb on + strand, within acs at 837.960 kb on + strand, within acs at 837.961 kb on - strand, within acs at 838.066 kb on + strand, within acs at 838.086 kb on - strand, within acs at 838.101 kb on + strand, within acs at 838.109 kb on - strand, within acs at 838.162 kb on + strand, within acs at 838.202 kb on + strand, within acs at 838.210 kb on - strand, within acs at 838.370 kb on + strand, within acs at 838.430 kb on + strand, within acs at 838.485 kb on + strand, within acs at 838.485 kb on + strand, within acs at 838.547 kb on + strand, within acs at 838.593 kb on - strand, within acs at 838.618 kb on + strand, within acs at 838.714 kb on + strand, within acs at 838.807 kb on - strand, within acs at 838.930 kb on - strand at 838.953 kb on + strand at 838.984 kb on + strand at 839.163 kb on - strand at 839.163 kb on - strand at 839.396 kb on - strand, within DVU0749 at 839.465 kb on - strand, within DVU0749 at 839.469 kb on + strand, within DVU0749 at 839.469 kb on + strand, within DVU0749 at 839.469 kb on + strand, within DVU0749 at 839.487 kb on - strand, within DVU0749 at 839.487 kb on - strand, within DVU0749 at 839.521 kb on + strand, within DVU0749 at 839.543 kb on + strand, within DVU0749 at 839.543 kb on + strand, within DVU0749 at 839.680 kb on + strand, within DVU0749 at 839.681 kb on + strand, within DVU0749 at 839.861 kb on - strand, within DVU0749 at 839.885 kb on + strand, within DVU0749 at 839.885 kb on + strand, within DVU0749 at 839.905 kb on - strand, within DVU0749 at 839.912 kb on - strand, within DVU0749 at 839.932 kb on + strand, within DVU0749 at 839.989 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with DMSO 7.5% remove 836,143 + cysW DVU0746 0.67 -0.1 836,372 + -1.8 836,709 + cysA DVU0747 0.51 -0.2 836,751 - cysA DVU0747 0.58 -0.1 836,838 + cysA DVU0747 0.71 +0.0 836,843 + cysA DVU0747 0.72 -0.6 836,871 + cysA DVU0747 0.77 -0.4 836,953 + cysA DVU0747 0.89 -0.2 837,048 - +1.3 837,118 - +0.1 837,136 - +0.2 837,145 + -0.2 837,228 - -0.1 837,268 - +0.5 837,276 + -1.5 837,287 + +1.3 837,430 - acs DVU0748 0.17 -0.0 837,787 + acs DVU0748 0.35 -1.8 837,810 + acs DVU0748 0.36 -1.3 837,818 - acs DVU0748 0.37 -0.9 837,826 + acs DVU0748 0.37 -0.6 837,905 + acs DVU0748 0.41 -2.3 837,905 + acs DVU0748 0.41 -3.6 837,907 + acs DVU0748 0.41 -2.1 837,960 + acs DVU0748 0.44 -1.1 837,961 - acs DVU0748 0.44 -2.3 838,066 + acs DVU0748 0.49 -3.6 838,086 - acs DVU0748 0.50 -1.4 838,101 + acs DVU0748 0.51 -2.3 838,109 - acs DVU0748 0.52 -1.6 838,162 + acs DVU0748 0.54 -1.9 838,202 + acs DVU0748 0.56 -2.7 838,210 - acs DVU0748 0.57 -2.6 838,370 + acs DVU0748 0.65 -1.6 838,430 + acs DVU0748 0.68 -2.4 838,485 + acs DVU0748 0.71 +0.7 838,485 + acs DVU0748 0.71 -2.6 838,547 + acs DVU0748 0.74 +0.3 838,593 - acs DVU0748 0.76 -2.4 838,618 + acs DVU0748 0.78 -1.3 838,714 + acs DVU0748 0.83 -2.3 838,807 - acs DVU0748 0.87 -0.3 838,930 - -1.7 838,953 + -1.0 838,984 + -1.5 839,163 - -0.1 839,163 - -1.0 839,396 - DVU0749 0.32 +0.2 839,465 - DVU0749 0.39 -0.0 839,469 + DVU0749 0.39 +0.1 839,469 + DVU0749 0.39 -1.0 839,469 + DVU0749 0.39 +0.1 839,487 - DVU0749 0.41 +0.3 839,487 - DVU0749 0.41 -0.7 839,521 + DVU0749 0.45 +0.7 839,543 + DVU0749 0.47 -0.0 839,543 + DVU0749 0.47 -0.5 839,680 + DVU0749 0.62 -1.3 839,681 + DVU0749 0.62 +0.1 839,861 - DVU0749 0.82 -0.4 839,885 + DVU0749 0.84 -0.5 839,885 + DVU0749 0.84 +0.9 839,905 - DVU0749 0.86 -0.8 839,912 - DVU0749 0.87 -0.5 839,932 + DVU0749 0.89 +0.4 839,989 + +0.1
Or see this region's nucleotide sequence