Experiment: MoYLS4 with DMSO 7.5%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wbaZ-2 and DVU0281 are separated by 104 nucleotides DVU0281 and mutY are separated by 239 nucleotides
DVU0280: wbaZ-2 - glycosyl transferase, group 1 family protein (TIGR), at 324,397 to 325,536
wbaZ-2
DVU0281: DVU0281 - exopolysaccharide biosynthesis protein, putative (TIGR), at 325,641 to 327,074
DVU0281
DVU0282: mutY - A/G-specific adenine glycosylase (TIGR), at 327,314 to 328,435
mutY
Position (kb) 325
326
327
328 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 324.712 kb on + strand, within wbaZ-2 at 324.712 kb on + strand, within wbaZ-2 at 324.824 kb on - strand, within wbaZ-2 at 324.868 kb on + strand, within wbaZ-2 at 324.982 kb on + strand, within wbaZ-2 at 325.131 kb on - strand, within wbaZ-2 at 325.189 kb on + strand, within wbaZ-2 at 325.196 kb on + strand, within wbaZ-2 at 325.221 kb on - strand, within wbaZ-2 at 325.221 kb on - strand, within wbaZ-2 at 325.223 kb on + strand, within wbaZ-2 at 325.235 kb on + strand, within wbaZ-2 at 325.310 kb on - strand, within wbaZ-2 at 325.310 kb on - strand, within wbaZ-2 at 325.567 kb on + strand at 325.896 kb on + strand, within DVU0281 at 326.087 kb on + strand, within DVU0281 at 326.104 kb on - strand, within DVU0281 at 326.242 kb on - strand, within DVU0281 at 326.284 kb on - strand, within DVU0281 at 326.783 kb on + strand, within DVU0281 at 326.796 kb on - strand, within DVU0281 at 326.895 kb on + strand, within DVU0281 at 327.011 kb on + strand at 327.130 kb on - strand at 327.312 kb on + strand at 327.319 kb on + strand at 327.319 kb on + strand at 327.320 kb on - strand at 327.327 kb on - strand at 327.337 kb on - strand at 327.337 kb on - strand at 327.379 kb on + strand at 327.387 kb on - strand at 327.445 kb on + strand, within mutY at 327.453 kb on - strand, within mutY at 327.487 kb on - strand, within mutY at 327.494 kb on + strand, within mutY at 327.499 kb on + strand, within mutY at 327.547 kb on - strand, within mutY at 327.669 kb on - strand, within mutY at 327.722 kb on - strand, within mutY at 327.743 kb on - strand, within mutY at 327.752 kb on + strand, within mutY at 327.757 kb on + strand, within mutY at 327.760 kb on - strand, within mutY at 327.877 kb on - strand, within mutY at 327.908 kb on - strand, within mutY at 327.960 kb on - strand, within mutY at 327.976 kb on + strand, within mutY at 327.978 kb on + strand, within mutY at 328.025 kb on + strand, within mutY at 328.071 kb on - strand, within mutY
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with DMSO 7.5% remove 324,712 + wbaZ-2 DVU0280 0.28 -0.1 324,712 + wbaZ-2 DVU0280 0.28 +0.2 324,824 - wbaZ-2 DVU0280 0.37 +0.2 324,868 + wbaZ-2 DVU0280 0.41 -2.1 324,982 + wbaZ-2 DVU0280 0.51 -1.0 325,131 - wbaZ-2 DVU0280 0.64 -2.8 325,189 + wbaZ-2 DVU0280 0.69 -1.7 325,196 + wbaZ-2 DVU0280 0.70 +0.9 325,221 - wbaZ-2 DVU0280 0.72 -0.2 325,221 - wbaZ-2 DVU0280 0.72 +0.1 325,223 + wbaZ-2 DVU0280 0.72 -2.1 325,235 + wbaZ-2 DVU0280 0.74 -1.0 325,310 - wbaZ-2 DVU0280 0.80 +0.0 325,310 - wbaZ-2 DVU0280 0.80 -0.0 325,567 + -0.2 325,896 + DVU0281 0.18 -0.9 326,087 + DVU0281 0.31 -0.3 326,104 - DVU0281 0.32 -1.4 326,242 - DVU0281 0.42 -1.8 326,284 - DVU0281 0.45 +1.2 326,783 + DVU0281 0.80 -2.4 326,796 - DVU0281 0.81 -0.4 326,895 + DVU0281 0.87 -1.5 327,011 + -2.0 327,130 - +0.1 327,312 + -1.6 327,319 + +0.1 327,319 + +0.5 327,320 - -0.2 327,327 - -0.8 327,337 - -2.1 327,337 - -0.9 327,379 + -0.8 327,387 - +0.4 327,445 + mutY DVU0282 0.12 +0.0 327,453 - mutY DVU0282 0.12 -0.9 327,487 - mutY DVU0282 0.15 -0.5 327,494 + mutY DVU0282 0.16 -0.1 327,499 + mutY DVU0282 0.16 +0.1 327,547 - mutY DVU0282 0.21 +0.1 327,669 - mutY DVU0282 0.32 -0.7 327,722 - mutY DVU0282 0.36 +0.1 327,743 - mutY DVU0282 0.38 -1.0 327,752 + mutY DVU0282 0.39 +0.4 327,757 + mutY DVU0282 0.39 -0.2 327,760 - mutY DVU0282 0.40 -1.7 327,877 - mutY DVU0282 0.50 -0.7 327,908 - mutY DVU0282 0.53 -1.0 327,960 - mutY DVU0282 0.58 +0.1 327,976 + mutY DVU0282 0.59 +0.4 327,978 + mutY DVU0282 0.59 -0.4 328,025 + mutY DVU0282 0.63 -0.0 328,071 - mutY DVU0282 0.67 -0.9
Or see this region's nucleotide sequence