Experiment: MoYLS4 with DMSO 10%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0349 and spsF are separated by 36 nucleotides spsF and DVU0351 overlap by 4 nucleotides DVU0351 and lpsC are separated by 7 nucleotides
DVU0349: DVU0349 - NeuB family protein (TIGR), at 396,098 to 397,156
DVU0349
DVU0350: spsF - spore coat polysaccharide biosynthesis protein spsF (TIGR), at 397,193 to 397,876
spsF
DVU0351: DVU0351 - cytidine 5monophosphate N-acetylneuraminic acid synthetase, putative/polysaccharide biosynthesis protein (TIGR), at 397,873 to 399,507
DVU0351
DVU0352: lpsC - aminotransferase, DegT/DnrJ/EryC1/StrS family (TIGR), at 399,515 to 400,696
lpsC
Position (kb) 397
398
399
400 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 396.959 kb on + strand, within DVU0349 at 396.961 kb on + strand, within DVU0349 at 396.998 kb on - strand, within DVU0349 at 397.070 kb on + strand at 397.092 kb on + strand at 397.092 kb on + strand at 397.174 kb on - strand at 397.190 kb on + strand at 397.250 kb on + strand at 397.259 kb on + strand at 397.289 kb on - strand, within spsF at 397.317 kb on + strand, within spsF at 397.326 kb on - strand, within spsF at 397.341 kb on - strand, within spsF at 397.428 kb on + strand, within spsF at 397.428 kb on + strand, within spsF at 397.481 kb on - strand, within spsF at 397.481 kb on - strand, within spsF at 397.517 kb on + strand, within spsF at 397.529 kb on + strand, within spsF at 397.568 kb on - strand, within spsF at 397.568 kb on - strand, within spsF at 397.569 kb on + strand, within spsF at 397.581 kb on + strand, within spsF at 397.598 kb on - strand, within spsF at 397.608 kb on - strand, within spsF at 397.659 kb on + strand, within spsF at 397.671 kb on + strand, within spsF at 397.677 kb on - strand, within spsF at 397.720 kb on + strand, within spsF at 397.813 kb on + strand at 397.847 kb on - strand at 397.869 kb on - strand at 397.898 kb on + strand at 397.913 kb on + strand at 397.921 kb on + strand at 398.020 kb on + strand at 398.024 kb on + strand at 398.031 kb on - strand at 398.151 kb on + strand, within DVU0351 at 398.151 kb on + strand, within DVU0351 at 398.152 kb on + strand, within DVU0351 at 398.195 kb on - strand, within DVU0351 at 398.257 kb on - strand, within DVU0351 at 398.287 kb on - strand, within DVU0351 at 398.295 kb on + strand, within DVU0351 at 398.516 kb on + strand, within DVU0351 at 398.630 kb on + strand, within DVU0351 at 398.638 kb on - strand, within DVU0351 at 398.671 kb on + strand, within DVU0351 at 398.824 kb on - strand, within DVU0351 at 398.824 kb on - strand, within DVU0351 at 398.829 kb on - strand, within DVU0351 at 398.975 kb on - strand, within DVU0351 at 398.985 kb on - strand, within DVU0351 at 398.990 kb on - strand, within DVU0351 at 399.008 kb on + strand, within DVU0351 at 399.013 kb on + strand, within DVU0351 at 399.016 kb on - strand, within DVU0351 at 399.041 kb on - strand, within DVU0351 at 399.141 kb on + strand, within DVU0351 at 399.240 kb on - strand, within DVU0351 at 399.285 kb on + strand, within DVU0351 at 399.293 kb on - strand, within DVU0351 at 399.463 kb on + strand at 399.484 kb on - strand at 399.580 kb on + strand at 399.598 kb on - strand at 399.698 kb on + strand, within lpsC at 399.698 kb on + strand, within lpsC at 399.708 kb on + strand, within lpsC at 399.713 kb on + strand, within lpsC at 399.713 kb on + strand, within lpsC at 399.713 kb on + strand, within lpsC at 399.721 kb on - strand, within lpsC at 399.721 kb on - strand, within lpsC at 399.787 kb on + strand, within lpsC at 399.788 kb on - strand, within lpsC at 399.888 kb on - strand, within lpsC at 399.911 kb on - strand, within lpsC at 399.913 kb on + strand, within lpsC at 400.085 kb on - strand, within lpsC at 400.102 kb on - strand, within lpsC at 400.139 kb on - strand, within lpsC at 400.187 kb on - strand, within lpsC at 400.204 kb on - strand, within lpsC at 400.209 kb on + strand, within lpsC at 400.209 kb on + strand, within lpsC at 400.217 kb on - strand, within lpsC at 400.217 kb on - strand, within lpsC at 400.243 kb on + strand, within lpsC at 400.262 kb on + strand, within lpsC at 400.314 kb on - strand, within lpsC at 400.317 kb on + strand, within lpsC at 400.386 kb on - strand, within lpsC at 400.389 kb on + strand, within lpsC at 400.407 kb on - strand, within lpsC at 400.422 kb on + strand, within lpsC at 400.427 kb on + strand, within lpsC at 400.454 kb on + strand, within lpsC at 400.490 kb on - strand, within lpsC
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with DMSO 10% remove 396,959 + DVU0349 0.81 -0.2 396,961 + DVU0349 0.81 -0.3 396,998 - DVU0349 0.85 -2.2 397,070 + -2.0 397,092 + -1.5 397,092 + -2.9 397,174 - -0.7 397,190 + +0.2 397,250 + -0.4 397,259 + -0.9 397,289 - spsF DVU0350 0.14 -1.7 397,317 + spsF DVU0350 0.18 -0.3 397,326 - spsF DVU0350 0.19 -0.7 397,341 - spsF DVU0350 0.22 +0.1 397,428 + spsF DVU0350 0.34 +0.1 397,428 + spsF DVU0350 0.34 -0.4 397,481 - spsF DVU0350 0.42 -0.4 397,481 - spsF DVU0350 0.42 -0.0 397,517 + spsF DVU0350 0.47 -0.3 397,529 + spsF DVU0350 0.49 +1.1 397,568 - spsF DVU0350 0.55 -0.8 397,568 - spsF DVU0350 0.55 +0.2 397,569 + spsF DVU0350 0.55 +0.1 397,581 + spsF DVU0350 0.57 -0.5 397,598 - spsF DVU0350 0.59 -1.3 397,608 - spsF DVU0350 0.61 +0.0 397,659 + spsF DVU0350 0.68 -0.1 397,671 + spsF DVU0350 0.70 -1.0 397,677 - spsF DVU0350 0.71 -0.5 397,720 + spsF DVU0350 0.77 -0.7 397,813 + -0.4 397,847 - +0.1 397,869 - +0.6 397,898 + -0.0 397,913 + -0.3 397,921 + -2.0 398,020 + -1.2 398,024 + -2.1 398,031 - -2.7 398,151 + DVU0351 0.17 -0.6 398,151 + DVU0351 0.17 -1.0 398,152 + DVU0351 0.17 -3.8 398,195 - DVU0351 0.20 -1.3 398,257 - DVU0351 0.23 -1.5 398,287 - DVU0351 0.25 -0.7 398,295 + DVU0351 0.26 -1.8 398,516 + DVU0351 0.39 -0.2 398,630 + DVU0351 0.46 -0.8 398,638 - DVU0351 0.47 -1.0 398,671 + DVU0351 0.49 -0.8 398,824 - DVU0351 0.58 -1.6 398,824 - DVU0351 0.58 -2.5 398,829 - DVU0351 0.58 -1.5 398,975 - DVU0351 0.67 +0.3 398,985 - DVU0351 0.68 -1.3 398,990 - DVU0351 0.68 -1.0 399,008 + DVU0351 0.69 -0.9 399,013 + DVU0351 0.70 -0.7 399,016 - DVU0351 0.70 -2.4 399,041 - DVU0351 0.71 -1.1 399,141 + DVU0351 0.78 -1.8 399,240 - DVU0351 0.84 -1.7 399,285 + DVU0351 0.86 -0.9 399,293 - DVU0351 0.87 -1.8 399,463 + -0.7 399,484 - -1.2 399,580 + -2.7 399,598 - -1.3 399,698 + lpsC DVU0352 0.15 -1.5 399,698 + lpsC DVU0352 0.15 -2.2 399,708 + lpsC DVU0352 0.16 -2.2 399,713 + lpsC DVU0352 0.17 -1.6 399,713 + lpsC DVU0352 0.17 -1.1 399,713 + lpsC DVU0352 0.17 -2.2 399,721 - lpsC DVU0352 0.17 +2.1 399,721 - lpsC DVU0352 0.17 -1.3 399,787 + lpsC DVU0352 0.23 -1.2 399,788 - lpsC DVU0352 0.23 -0.9 399,888 - lpsC DVU0352 0.32 -1.0 399,911 - lpsC DVU0352 0.34 -0.9 399,913 + lpsC DVU0352 0.34 -2.2 400,085 - lpsC DVU0352 0.48 -1.2 400,102 - lpsC DVU0352 0.50 -1.5 400,139 - lpsC DVU0352 0.53 -1.2 400,187 - lpsC DVU0352 0.57 -1.1 400,204 - lpsC DVU0352 0.58 -1.6 400,209 + lpsC DVU0352 0.59 -2.2 400,209 + lpsC DVU0352 0.59 -0.2 400,217 - lpsC DVU0352 0.59 -1.1 400,217 - lpsC DVU0352 0.59 -1.3 400,243 + lpsC DVU0352 0.62 -3.4 400,262 + lpsC DVU0352 0.63 -1.2 400,314 - lpsC DVU0352 0.68 -0.9 400,317 + lpsC DVU0352 0.68 -1.6 400,386 - lpsC DVU0352 0.74 -2.9 400,389 + lpsC DVU0352 0.74 -1.9 400,407 - lpsC DVU0352 0.75 -2.1 400,422 + lpsC DVU0352 0.77 -1.1 400,427 + lpsC DVU0352 0.77 -1.8 400,454 + lpsC DVU0352 0.79 -1.2 400,490 - lpsC DVU0352 0.82 -0.6
Or see this region's nucleotide sequence